Implications of pleiotrophin in human PC3 prostate cancer cell growth in vivo
| Autor: | Maria Hatziapostolou, Margarita Lamprou, Panagiota Ravazoula, Kostas Dimas, Evangelia Papadimitriou, Sotiria Tsirmoula |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Angiogenesis medicine.medical_treatment Blotting Western Transplantation Heterologous Fluorescent Antibody Technique Apoptosis Mice SCID Biology Pleiotrophin Transfection Metastasis Prostate cancer Mice In vivo Cell Movement Mice Inbred NOD Internal medicine Cell Line Tumor medicine Cell Adhesion In Situ Nick-End Labeling Animals Humans Growth factor Cancer Prostatic Neoplasms General Medicine Neoplasms Experimental Original Articles medicine.disease Endocrinology Oncology Cancer cell Cancer research Cytokines Carrier Proteins |
| Zdroj: | Cancer Sci |
| ISSN: | 1349-7006 |
| Popis: | Pleiotrophin (PTN) is a heparin-binding growth factor with diverse functions related to tumor growth, angiogenesis, and metastasis. Pleiotrophin seems to have a significant role in prostate cancer cell growth and to mediate the stimulatory actions of other factors that affect prostate cancer cell functions. However, all studies carried out up to date are in vitro, using different types of human prostate cancer cell lines. The aim of the present work was to study the role of endogenous PTN in human prostate cancer growth in vivo. For this purpose, human prostate cancer PC3 cells were stably transfected with a plasmid vector, bearing the antisense PTN sequence, in order to inhibit PTN expression (AS-PC3). Migration, apoptosis, and adhesion on osteoblastic cells were measured in vitro. In vivo, PC3 cells were s.c. injected into male NOD/SCID mice, and tumor growth, survival rates, angiogenesis, apoptosis, and the number of metastasis were estimated. Pleiotrophin depletion resulted in a decreased migration capability of AS-PC3 cells compared with the corresponding mock-transfected or the non-transfected PC3 cells, as well as increased apoptosis and decreased adhesiveness to osteoblastic cells in vitro. In prostate cancer NOD/SCID mouse xenografts, PTN depletion significantly suppressed tumor growth and angiogenesis and induced apoptosis of cancer cells. In addition, PTN depletion decreased the number of metastases, providing a survival benefit for the animals bearing AS-PC3 xenografts. Our data suggest that PTN is implicated in human prostate cancer growth in vivo and could be considered a potential target for the development of new therapeutic approaches for prostate cancer. |
| Databáze: | OpenAIRE |
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