Cytokine-inducible SH2 protein (CIS3) and JAK2 binding protein (JAB) abolish prolactin receptor-mediated STAT5 signaling

Autor: Shoshana Merchav, Akihiko Yoshimura, Yael Cohen, Akira Matsumoto, Yael Sandowski, Arieh Gertler, Daniel Helman
Rok vydání: 1998
Předmět:
Protein family
Receptors
Prolactin
Biophysics
Suppressor of Cytokine Signaling Proteins
Biochemistry
Cell Line
src Homology Domains
Mice
Mammary Glands
Animal
Suppressor of Cytokine Signaling 1 Protein
Structural Biology
STAT5 Transcription Factor
Genetics
Animals
Humans
Luciferase
Cytokine-inducible SH2 protein
JAK2 binding protein
Molecular Biology
STAT5
Inhibition
Cytokine signaling
Reporter gene
biology
Binding protein
Prolactin receptor
Postpartum Period
Intracellular Signaling Peptides and Proteins
Proteins
Cell Biology
Milk Proteins
Placental Lactogen
Molecular biology
Recombinant Proteins
Rats
DNA-Binding Proteins
Repressor Proteins
Suppressor of Cytokine Signaling 3 Protein
Trans-Activators
biology.protein
Cattle
Female
Signal transduction
Carrier Proteins
Postpartum period
Signal Transduction
Transcription Factors
Zdroj: FEBS Letters. 441:287-291
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(98)01555-5
Popis: The ability of five members of the cytokine-inducible SH2 protein family (CIS1–4) and JAK2 binding (JAB) protein to affect prolactin receptor (PRLR)-mediated activity was tested in human 293 embryonic kidney fibroblasts transiently transfected with rat PRLR, five concentrations of CIS/JAB Myc-tagged cDNAs and a STAT5-responsive reporter gene encoding luciferase. The protein expressions of CIS1, CIS2, CIS3 and JAB were comparable, whereas the level of CIS4 was slightly lower. PRLR-mediated luciferase activity was abolished in a dose-dependent manner in cells transfected with cDNA of CIS3 or JAB, even at concentrations below the level of protein detection by anti-Myc antibody. In contrast, CIS1, CIS2 and CIS4 had little or no effect, despite similar levels of expression. CIS1 expression in postpartum mouse mammary glands was high and changed little in the course of 3 days. CIS2 and CIS3 expression was also high and increased further, whereas JAB expression was very low. These results hint that at least in mammary gland CIS3 is likely the main physiological negative regulator of the PRLR-mediated JAK2/STAT5 pathway.
Databáze: OpenAIRE
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