Métodos de identificación molecular para Gluconacetobacter diazotrophicus

Autor: Molinares-Pacheco, Cristian, Muñoz-Morales, Julieta Mariana, Carbajal-Armenta, Adriana, Hernández-Tenorio, Ana Laura, Cueto-Becerra, Alejandro, Muñoz-Rojas, Jesús
Jazyk: Spanish; Castilian
Rok vydání: 2021
Předmět:
DOI: 10.5281/zenodo.5104709
Popis: RESUMEN Los métodos para la identificación de microorganismos se valen de sus características morfológicas, fisiológicas, proteómicas y genómicas. En la identificación tradicional se emplean técnicas dependientes de cultivo que pueden presentar inconvenientes según el tipo de microorganismo a identificar, su capacidad de crecimiento, su parecido con algún otro microbio y el tiempo total para su identificación. El uso de PGPB en la agricultura ha repercutido positivamente reduciendo costos de producción, disminuyendo el impacto negativo sobre el medio ambiente y la salud humana. G. diazotrophicus es una bacteria diazótrofo-endofítica con características de PGPB que tiene un tiempo de crecimiento más extenso en comparación con otras bacterias, y presenta características fenotípicas y genéticas similares a bacterias fijadoras de nitrógeno de su mismo género, por tanto, su identificación empleando únicamente métodos tradicionales puede ser algo laboriosa e inespecífica. En la presente revisión se analizaron algunos de los métodos de identificación molecular utilizados para G. diazotrophicus. ABSTRACT The methods for identifying microorganisms are based on their morphological, physiological, proteomic, and genomic characteristics. Under traditional identification, culture-dependent techniques are used, but they may present drawbacks depending on the type of microorganism to be identified, its growth capacity, its resemblance to some other microbe and the total time for its identification. The use of PGPB in agriculture has had a positive impact by reducing production costs, reducing the negative impact on the environment and human health. G. diazotrophicus is a diazotrophic-endophytic bacterium with PGPB characteristics that have a longer growth time compared to other bacteria, and it presents phenotypic and genetic characteristics like nitrogen-fixing bacteria of the same genus, therefore, its identification using only traditional methods can be somewhat laborious and unspecific. In this review, some molecular methods for identification of G. diazotrophicus were analysed.
{"references":["Merchán MA, Torres Caicedo MI, Díaz Torres AK. Técnicas de Biología Molecular en el desarrollo de la investigación. Revisión de la literatura. Rev Habanera Ciencias Médicas. 2017;16(5).","Raina V, Nayak T, Ray L, Kumari K, Suar M. A Polyphasic Taxonomic Approach for Designation and Description of Novel Microbial Species [Internet]. Microbial Diversity in the Genomic Era. Elsevier Inc.; 2019. 137–152 p.","Pascual Hernández Á, Balleztero-Téllez M, Galán-Sanchez F, Rodríguez Iglesias M. Aplicación de la espectrometría de masas en la identificación de bacterias. In: Enfermedades infecciosas y Microbiología Clínica. 2016. p. 8–18.","Wajswol Martella S. Efecto de la aplicación de vinaza en cultivos de caña de azúcar. Estudio de la comunidad bacteriana. 2018.","Bou G, Fernández-Olmos A, Sáez-Nieto JA, Valdezate S. Métodos de identificación bacteriana en el laboratorio de microbiología. In: Enfermedades infecciosas y Microbiología Clínica. 2011. p. 601–8.","Morales-García YE, Juárez-Hernández D, Aragón-Hernández C, Mascarua-Esparza MA, Bustillos-Cristales MR, Fuentes-Ramírez LE, et al. Growth response of maize plantlets inoculated with Enterobacter spp., as a model for alternative agriculture. Rev Argent Microbiol [Internet]. 2011;43(4):287–93.","Rilling JI, Acuña JJ, Nannipieri P, Cassan F, Maruyama F, Jorquera MA. Current opinion and perspectives on the methods for tracking and monitoring plant growth‒promoting bacteria. Soil Biol Biochem. 2019;130:205–19.","Rocha G, Le Queré A, Medina A, Cuéllar A, Contreras J-L, Carreño R, et al. Diversity and phenotypic analyses of salt‑ and heat‑tolerant wild bean.pdf. Arch Microbiol. 2019;16.","Molina-Romero D, Baez A, Quintero-Hernández V, Castañeda-Lucio M, Fuentes-Ramírez LE, Bustillos-Cristales M del R, et al. Compatible bacterial mixture, tolerant to desiccation, improves maize plant growth. PLoS One. 2017;12(11):1–21.","Meza RFR. Biodiversidad de ecotipos de Moniliophthora roreri en cacao (Theobroma cacao) clon ccn-51 y la actividad antagonista de PGPR [Internet]. 2018.","Olanrewaju OS, Glick BR, Babalola OO. Mechanisms of action of plant growth promoting bacteria. World J Microbiol Biotechnol [Internet]. 2017;33(11):0.","Rivera-Urbalejo AP, Rodríguez-Andrade O, Morales-García YE, Quintero-Hernández V, Muñoz-Morales JM, Carbajal-Armenta A, et al. Inoculación de plántulas micropropagadas de caña de azúcar con bacterias benéficas para potenciar su producción. Alianzas y Tendencias BUAP [Internet]. 2017;2(7):15–26.","Pazos-Rojas LA, Marín-Cevada V, Elizabeth Y, García M, Baez A., et al. Uso de microorganismos benéficos para reducir los daños causados por la revolución verde. Rev Iberoam Ciencias. 2016;3(7):72–85.","Vivanco-Calixto R, Molina-Romero D, Morales-García YE, Quintero-Hernández V, Munive-Hernández A, Baez-Rogelio A, et al. Reto agrobiotecnológico: inoculantes bacterianos de segunda generación. Alianzas y Tendencias [Internet]. 2016;1(April):9–19.","Molina Romero D. Diseño de inoculante de segunda generación tolerante a condiciones de desecación. Benemérita Universidad Autónoma de Puebla; 2017.","Muñoz Rojas J, Caballero Mellado J. Gluconacetobacter diazotrophicus, modelo de bacteria endófita. Microbios en línea. 2001;157–76.","Paredes-Villanueva J, Del Rosario J, Urcia M, Zavaleta-Armas J. Plant growth promoter collection of Gluconacetobacter diazotrophicus from the northern coast of Peru. Sci Agropecu. 2020;11(1):15–21.","Saravanan V, Madhaiyan M, Osborne J, Thangaraju M, Sa T. Ecological Occurrence of Gluconacetobacter diazotrophicus and Nitrogen-fixing Acetobacteraceae Members: Their Possible Role in Plant Growth Promotion. Microb Ecol. 2008;55(1):130–40.","Ahmad I, Altaf M, Sharma J, Al-thubiani A. Diversity, Quorum Sensing, and Plant Growth Promotion by Endophytic Diazotrophs Associated with Sugarcane with Special Reference to Gluconacetobacter diazotrophicus. Plant-Microbe Interact An Approach to Sustain Agric. 2016;(495–509).","Silva R, Filgueiras L, Santos B, Coelho M, Silva M, Estrada-Bonilla G, et al. Gluconacetobacter diazotrophicus Changes The Molecular Mechanisms of Root Development in Oryza sativa L. Growing Under Water Stress. Int J Mol Sci. 2020;21(1):333.","Trcek J, Barja F. Updates on quick identification of acetic acid bacteria with a focus on the 16S–23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDI-TOF mass spectrometry. Int J Food Microbiol. 2015;196:137–44.","Cleenwerck I, De Wachter M, González Á, De Vuyst L, De Vos P. Differentiation of species of the family Acetobacteraceae by AFLP DNA fingerprinting: Gluconacetobacter kombuchae is a later heterotypic synonym of Gluconacetobacter hansenii. Int J Syst Evol Microbiol. 2009;58(7):1771–86.","Sabat A, van Zanten E, Akkerboom V, Wisselink G, van Slochteren K, de Boer R, et al. Targeted next-generation sequencing of the 16S-23S rRNA region for culture-independent bacterial identification-increased discrimination of closely related species. Sci Rep. 2017;7(1):1–12.","Fernández Olmos A, García de la Fuente C, Sáez Nieto JA, Valdezate Ramos S. Métodos de identificación bacteriana en el laboratorio de microbiología. Procedimientos en Microbiol Clínica. 2010;5–9.","Rodicio M del R, Mendoza M del C. Identificación bacteriana mediante secuenciación del ARNr 16S: fundamento, metodología y aplicaciones en microbiología clínica. Enferm Infecc Microbiol Clin. 2004;22:238–45.","Law J, Tan K, Wong S, Ab Mutalib N, Lee L. Taxonomic and Characterization Methods of Streptomyces: A Review. Prog Microbes Mol Biol. 2018;1(1).","Adékambi T, Drancourt M, Raoult D. The rpoB gene as a tool for clinical microbiologists. Trends Microbiol. 2009;17(1):37–45.","Kenna D, Fuller A, Martin K, Perry C, Pike R, Burns P, et al. rpoB gene sequencing highlights the prevalence of an E. miricola cluster over other Elizabethkingia species among UK cystic fibrosis patients. Diagn Microbiol Infect Dis. 2018;90(2):109–14.","Pandey C, Neigi Y, Maheshwari D, Rawat D, Prabha D. Potential of native cold tolerant plant growth promoting bacilli to enhance nutrient use efficiency and yield of Amaranthus hypochondriacus. Plant Soil. 2018;428(1–2):307–20.","Bunpa S, Nishibuchi M, Thawonsuwan J, Sermwittayawong N. Genetic heterogeneity among Vibrio alginolyticus strains, and design of a PCR-based identification method using gyrB gene sequence. Can J Microbiol. 2018;64(1):1–10.","Cox MM. Regulation of bacterial RecA protein function. Crit Rev Biochem Mol Biol. 2007;42(1):41–63.","Mahenthiralingam E, Bischof J, Byrne SK, Radomski C, Davies JE, Av-Gay Y, et al. DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. Vol. 38, Journal of Clinical Microbiology. 2000. p. 3165–73.","Proença D, Schwab S, Baldani J, Morais P. Diversity and function of endophytic microbial community of plants with economical potential. In: Diversity and Benefits of Microorganisms from the Tropics. 2017. p. 209–43.","Wang W, Zhai Y, Cao L, Tan H, R Z. Endophytic bacterial and fungal microbiota in sprouts, roots and stems of rice (Oryza sativa L.). Microbiol Res. 2016;188–9.","Huang Y. Illumina-based Analysis of Endophytic Bacterial Diversity of four Allium species. Sci Rep. 2019;9(1):1–11.","Zhang W, Wang X, Qi X, Ren 'L, Qiang T. Isolation and identification of a bacterial cellulose synthesizing strain from kombucha in different conditions: Gluconacetobacter xylinus ZHCJ618. Food Sci Biotechnol. 2018;27(3):705–13.","Numata Y, Kono H, Mori A, Kishimoto R, Tajima K. Structural and rheological characterization of bacterial cellulose gels obtained from Gluconacetobacter genus. Food Hydrocoll. 2019;92:233–9.","Torija M, Mateo E, Mas A. Identification and quantification of acetic acid bacteria in wine and vinegar by TaqMan–MGB probes. Food Microbiol. 2010;27(2):257–65.","Eskin N, Vessey K, Tian L. Research progress and perspectives of nitrogen fixing bacterium, Gluconacetobacter diazotrophicus, in monocot plants. Int J Agron. 2014;2014.","Ruiz A, Poblet M, Mas A, Guillamón J. Identification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer. Int J Syst Evol Microbiol. 2000;50(6):1981–7.","Arencibia A, Vinagre F, Estevez Y, Bernal A, Perez J, Cavalcanti J, et al. Gluconoacetobacter diazotrophicus elicitate a sugarcane defense response against a pathogenic bacteria Xanthomonas albilineans. Plant Signal Behav. 2006;1(5):265–73.","Casas M, Pérez J, Piñón D, Bernal A, de los Ángeles Zadrón M, Estrada M, et al. Colonización e interacción del endófito Gluconacetobacter diazotrophicus en vitro plantas de caña de azúcar. Rev Cuba Caña. 2015;(1):26–32.","Arencibia A, Bernal A, Yang L, Cortegaza L, Carmona E, Perez A, et al. New role of phenylpropanoid compounds during sugarcane micropropagation in Temporary Immersion Bioreactors (TIBs). Plant Sci. 2008;175(4):487–96.","Mehnaz S, Weselowski B, Lazarovits G. Isolation and identification of Gluconacetobacter azotocaptans from corn rhizosphere. Syst Appl Microbiol. 2006;29(6):496–501.","Trcek J. Quick identification of acetic acid bacteria based on nucleotide sequences of the 16S–23S rDNA internal transcribed spacer region and of the PQQ-dependent alcohol dehydrogenase gene. Syst Appl Microbiol. 2005;28(8):735–45.","Domínguez M, Coll P, Coque T, Vázquez J, Villa J. Métodos moleculares de tipificación epidemiológica en bacteriología. Procedimientos en Microbiol Clínica. 2005;9–31.","Morales García YE, Rodríguez-Andrade O, Hernández-Tenorio A, Juárez Hernández D, Castañeda-Antonio D, Molina-Romero D, et al. Caracterización molecular de cepas contenidas en un inoculante multiespecies. In: XI encuentro Participación de la Mujer en la Ciencia. 2014. p. 5.","Guedes H, Santos S, Perin L, Teixeira K, Reis V, Baldani J. Polyphasic characterization of Gluconacetobacter diazotrophicus isolates obtained from different sugarcane varieties. Brazilian J Microbiol. 2008;39(4):718–23.","De Vuyst L, Camu N, De Winter T, Vandemeulebroecke K, Van de Perre V, Vancanneyt M, et al. Validation of the (GTG) 5-rep-PCR fingerprinting technique for rapid classification and identification of acetic acid bacteria, with a focus on isolates from Ghanaian fermented cocoa beans. Int J Food Microbiol. 2008;125(1):79–90."]}
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