Respiratory and GIT tract immune responses of broiler chickens following experimental infection with Newcastle disease’s virus
| Autor: | Hadi Rohollahzadeh, Amir Hossein Asl Najjari, Saeed Mokhayeri, Keramat Asasi, H. Nili |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cellular immunity Immunohystochemistry Newcastle disease Virus Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Immune system Intestinal mucosa T lymphocyte Medicine biology business.industry Broiler biology.organism_classification 030104 developmental biology Immunology Original Article Anatomy business CD8 Lymphoproliferative response 030215 immunology |
| Zdroj: | Comparative Clinical Pathology |
| ISSN: | 1618-565X 1618-5641 |
| Popis: | Newcastle disease causes a lymphoproliferative response in the tracheal and intestinal mucosa of the infected birds. In this study, the Hitchner B1 and I-2 vaccine and challenging of ND field strains were used to evaluate the populations of T lymphocyte subsets infiltrated intestinal and tracheal, also to shed some light on cell-mediated immune response using enzyme-linked immunosorbent assay (ELISA) detecting chicken’s serum interferon-γ. Three hundred-day-old broilers were randomly divided into four groups. Groups 1 and 2 received I-2 and B1 vaccines, respectively, while groups 3 and 4 were challenged-unvaccinated and unchallenged-unvaccinated groups. Blood samples were taken from five random chicks and were then tested with ELISA test. Three chicks of each group were euthanized after vaccine administration and also challenging with acute virus. Interferon-γ changes were significant in time (p |
| Databáze: | OpenAIRE |
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