Failure Mechanisms in DNA Self-Assembly: Barriers to Single-Fold Yield
| Autor: | Michael Zwolak, J. Alexander Liddle, Anthony J. Kearsley, Paul N. Patrone, Jacob M. Majikes |
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| Rok vydání: | 2021 |
| Předmět: |
Scaffold
General Engineering General Physics and Astronomy 02 engineering and technology DNA 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Nanostructures Folding (chemistry) chemistry.chemical_compound chemistry Yield (chemistry) Biophysics Nucleic acid DNA origami Nanotechnology Nucleic Acid Conformation General Materials Science Self-assembly 0210 nano-technology Function (biology) |
| Zdroj: | ACS nano. 15(2) |
| ISSN: | 1936-086X |
| Popis: | Understanding the folding process of DNA origami is a critical stepping stone to the broader implementation of nucleic acid nanofabrication technology but is notably nontrivial. Origami are formed by several hundred cooperative hybridization events-folds-between spatially separate domains of a scaffold, derived from a viral genome, and oligomeric staples. Individual events are difficult to detect. Here, we present a real-time probe of the unit operation of origami assembly, a single fold, across the scaffold as a function of hybridization domain separation-fold distance-and staple/scaffold ratio. This approach to the folding problem elucidates a predicted but previously unobserved blocked state that acts as a limit on yield for single folds, which may manifest as a barrier in whole origami assembly. |
| Databáze: | OpenAIRE |
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