CD36 overexpression in ritonavir-treated THP-1 cells is reversed by α-tocopherol
| Autor: | Adelina Munteanu, Angelo Azzi, Roberta Ricciarelli, Jean-Marc Zingg |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
CD36 Antigens
CD36 medicine.medical_treatment alpha-Tocopherol Pharmacology Biochemistry Monocytes Cell Line Downregulation and upregulation Physiology (medical) parasitic diseases medicine Vitamin E heterocyclic compounds THP1 cell line RNA Messenger Scavenger receptor DNA Primers Ritonavir Protease Base Sequence biology virus diseases hemic and immune systems HIV Protease Inhibitors Cholesterol Proteasome Proteasome inhibitor biology.protein lipids (amino acids peptides and proteins) Proteasome Inhibitors medicine.drug |
| Zdroj: | Free Radical Biology and Medicine. 38:1047-1056 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/j.freeradbiomed.2004.12.030 |
| Popis: | Therapies with antiretroviral protease inhibitors (ARPI) are correlated with a higher risk for dyslipidemia, hypercholesterolemia, and atherosclerosis. The original aim of this study was to establish whether alpha-tocopherol can reduce CD36 scavenger receptor overexpression occurring after treatment of monocytes with the ARPI ritonavir. We show here that treatment of THP-1 monocytes with ritonavir increases total protein and surface expression of CD36; however, only weak changes are observed at the mRNA level, suggesting that CD36 overexpression occurs mainly at the posttranscriptional level. Concentrations of ritonavir that upregulate CD36 expression inhibit proteasome activity in THP-1 cells, indicating a possible regulatory role of the proteasome in CD36 overexpression. Similar to ritonavir, the proteasome inhibitor ALLN increases the CD36 surface expression on THP-1 cells. alpha-Tocopherol efficiently normalizes CD36 protein overexpression after ritonavir treatment and reduces oxLDL uptake. Furthermore, in THP-1 monocytes, alpha-tocopherol reverses the proteasome activity inhibited by ritonavir. This study indicates that an increased CD36 protein expression in THP-1 monocytes induced by ritonavir can be normalized by alpha-tocopherol. CD36 overexpression is caused by inhibition of proteasome activity by ritonavir, which is efficiently restored by alpha-tocopherol. |
| Databáze: | OpenAIRE |
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