The Zinc-Responsive Regulator Zur Controls Expression of the Coelibactin Gene Cluster in Streptomyces coelicolor
| Autor: | Claire M. Strain-Damerell, Mark S. B. Paget, Dimitris Kallifidas, Ben Pascoe, Gillian A. Owen, Hee-Jeon Hong |
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| Rok vydání: | 2009 |
| Předmět: |
Genetics
biology Streptomyces coelicolor Mutant Repressor Gene Expression Regulation Bacterial biology.organism_classification Polymerase Chain Reaction Microbiology Phenotype Bacterial Proteins Multigene Family Gene cluster Gene expression Gene Regulation DNA microarray Molecular Biology Gene Oligonucleotide Array Sequence Analysis |
| Popis: | Streptomyces coelicolor mutants lacking the zinc-responsive Zur repressor are conditionally defective in sporulation, presumably due to the overexpression of one or more Zur target genes. Gene disruption analyses revealed that deregulation of previously known Zur targets was not responsible for the sporulation phenotype. We used microarrays to identify further Zur targets and discovered that Zur controls a cluster of genes predicted to direct synthesis of an uncharacterized siderophore-related non-ribosomally encoded peptide designated coelibactin. Disruption of a key coelibactin biosynthetic gene suppressed the Zur sporulation phenotype, suggesting that deregulation of coelibactin synthesis inhibits sporulation. |
| Databáze: | OpenAIRE |
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