Effects of NEFAs during IVM on pig embryos from granulosa cell-cocultured oocytes
Autor: | Meihong Shi, Marc-André Sirard |
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Rok vydání: | 2021 |
Předmět: |
Swine
Granulosa cell Embryonic Development Biology Fatty Acids Nonesterified Andrology Transcriptome 03 medical and health sciences 0302 clinical medicine Gene expression Genetics medicine Animals Blastocyst 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine Cumulus Cells Granulosa Cells Embryogenesis Embryo Cell Biology Oocyte In vitro maturation In Vitro Oocyte Maturation Techniques medicine.anatomical_structure embryonic structures Oocytes Female Developmental Biology |
Zdroj: | Molecular reproduction and developmentREFERENCES. 88(12) |
ISSN: | 1098-2795 |
Popis: | Circulating levels of nonesterified fatty acids (NEFAs) are elevated in some females, which can impair oocyte maturation and embryo development, and may alter the phenotype of the progeny. However, the effects of NEFAs on human embryo development are not clear due to ethical limitations. Thus, we used pig as the model to investigate the impacts of NEFAs on oocyte and embryo due to their similar reproductive and metabolic physiologies to humans. In this study, porcine cumulus-oocyte complexes were in vitro maturated under a pathologically high concentration of NEFAs (468 μM palmitic acid, 194 μM stearic acid, and 534 μM oleic acid) with the presence of granulosa cell monolayer, in contrast to control without NEFAs. The mature oocytes were fertilized to produce embryos for further analysis of the transcriptome and DNA methylation patterns. The elevated level of NEFAs decreased the blastocyst rate and delayed the blastocyst development. Ingenuity pathway analysis showed that the most affected gene pathways were related mainly to cell activities, metabolism, and inflammation. These findings indicated that oocytes exposed to the exogenous high level of NEFAs during in vitro maturation resulted in altered gene expression and DNA methylation of early embryos, which have detrimental impacts on blastocyst quality. |
Databáze: | OpenAIRE |
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