Antioxidant and developmental capacity of retinol on thein vitroculture of rabbit embryos
| Autor: | Mohamed F. Saad, H. M. Safaa, Ayman Moustafa Saeed, Ashraf M. El-Sayed, Gamal M. K. Mehaisen, Ahmed M. Elomda, Ahmed O. Abass |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Embryonic Development Fertilization in Vitro medicine.disease_cause Antioxidants Embryo Culture Techniques Superoxide dismutase Andrology 03 medical and health sciences chemistry.chemical_compound medicine Animals Blastocyst Vitamin A chemistry.chemical_classification biology Glutathione peroxidase Embryogenesis Embryo Vitamins Cell Biology Embryo Mammalian Malondialdehyde 030104 developmental biology medicine.anatomical_structure chemistry biology.protein Female Rabbits Oxidative stress Developmental Biology |
| Zdroj: | Zygote. 26:326-332 |
| ISSN: | 1469-8730 0967-1994 |
| Popis: | SummaryOxidative stress is a major cause of defective embryo development duringin vitroculture. Retinoids are recognized as non-enzymatic antioxidants and may have an important role in the regulation of cell differentiation and vertebrate development. However, there are not enough reports discussing the antioxidant and developmental capacity of retinoids, including retinol (RT), on thein vitrodevelopment of embryos recovered from livestock animals, particularly in rabbit species. Therefore, morula embryos obtained from nulliparous Red Baladi rabbit does were cultured for 48 h in TCM199 medium in the absence of RT (control group) or in the presence of RT at concentrations of 10, 100 and 1000 nM. The developmental capacity to the hatched blastocyst stage, the antioxidant biomarker assay and the expression of several selected genes were analyzed in each RT group. The data show that RT significantly (PPPGJA1), POU class 5 homeobox 1 (POU5F1) and superoxide dismutase 1 (SOD1) genes compared with the control group. The current study highlights the potential effects of RT as antioxidant in the culture medium on thein vitrodevelopment of rabbit embryos. |
| Databáze: | OpenAIRE |
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