Intravital Vascular Phototheranostics and Real-Time Circulation Dynamics of Micro- and Nano-sized Erythrocyte-Derived Carriers
| Autor: | Wangcun Jia, Ben Lertsakdadet, Bahman Anvari, Jack C. Tang, Betty Villantay, Bernard Choi, Joshua M. Burns, J. Stuart Nelson, Raviraj Vankayala |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Fluorescence-lifetime imaging microscopy Materials science Erythrocytes Inflammatory response Bioengineering 02 engineering and technology laser dermatologic surgery Physical Chemistry Article Theranostic Nanomedicine Macromolecular and Materials Chemistry 03 medical and health sciences Mice 0302 clinical medicine Engineering Neoplasms cancer Nanotechnology Animals General Materials Science Near infrared imaging Nanoscience & Nanotechnology Skin Drug Carriers Optical Imaging Single injection Chemical Engineering 021001 nanoscience & nanotechnology Nanostructures delivery systems near-infrared imaging 030220 oncology & carcinogenesis Chemical Sciences erythrocyte engineering 0210 nano-technology port wine stain Biomedical engineering phototherapy |
| Zdroj: | ACS Appl Mater Interfaces ACS applied materials & interfaces, vol 12, iss 1 |
| Popis: | Erythrocyte-based carriers can serve as theranostic platforms for delivery of imaging and therapeutic payloads. Engineering these carriers at micro- or nano-scales makes them potentially useful for broad clinical applications ranging from vascular diseases to tumor theranostics. Longevity of these carriers in circulation is important in delivering a sufficient amount of their payloads to the target. We have investigated the circulation dynamics of micro (~4.95 μm diameter) and nano (~91 nm diameter) erythrocyte-derived carriers in real time using near-infrared fluorescence imaging, and evaluated the effectiveness of such carrier systems in mediating photothermolysis of cutaneous vasculature in mice. Fluorescence emission half-lives of micro- and nano-sized carriers in response to a single intravenous injection were ~49 and ~15 minutes, respectively. A single injection of micro-sized carriers resulted in threefold increase in signal to noise ratio that remained nearly persistent over one hour of imaging time. Our results also suggest that a second injection of the carriers seven days later can induce a transient inflammatory response, as manifested by the apparent leakage of the carriers into the perivascular tissue. The administration of the carriers into the mice vasculature reduced the threshold laser fluence to induce photothermolysis of blood vessels from >65 to 20 J/cm(2). We discuss the importance of membrane physicochemical and mechanical characteristics in engineering erythrocyte-derived carriers, and considerations for their clinical translation. |
| Databáze: | OpenAIRE |
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