Radiolabeling, Characteristics and NanoSPECT/CT Imaging of 188Re-cetuximab in NCI-H292 Human Lung Cancer Xenografts
| Autor: | Wei-Chuan Hsu, Tsung-Tse Wu, Kai-Hung Cheng, Chih-Hsien Chang, Ya-Jen Chang, Liang-Cheng Chen, Wan-I Kuo |
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| Rok vydání: | 2018 |
| Předmět: |
Cancer Research
Lung Neoplasms Single Photon Emission Computed Tomography Computed Tomography medicine.medical_treatment Cell Cetuximab Mice 03 medical and health sciences 0302 clinical medicine Cell Line Tumor medicine Animals Humans Cytotoxic T cell Epidermal growth factor receptor neoplasms Cell Proliferation Radioisotopes biology business.industry Cancer General Medicine Radioimmunotherapy medicine.disease Xenograft Model Antitumor Assays digestive system diseases In vitro ErbB Receptors Rhenium medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research biology.protein Antibody business medicine.drug |
| Zdroj: | Anticancer Research. 39:183-190 |
| ISSN: | 1791-7530 0250-7005 |
| DOI: | 10.21873/anticanres.13096 |
| Popis: | Background/aim Cetuximab has exhibited high EGFR-targeting specificity and clinical promise in previous studies. In this study, we formulated unit dose kits for preparation of high specific activity 188Re-cetuximab for imaging and treatment of EGFR-positive cancer. Materials and methods 188Re-cetuximab was prepared by adding 0.37-0.74 GBq/0.5 ml of 188Re-perrhenate for 4 h at 37°C. Cell surface expression of EGFR, cell binding and cytotoxic effects were evaluated in vitro using both EGFR-positive (NCI-H292, A431) and EGFR-negative (BT483) tumors. A nanoSPECT/CT imaging study was performed in mice bearing EGFR-expressing NCI-H292 tumors. Results 188Re-cetuximab bound specifically to EGFR-expressing cells and labeling of radionuclides to cetuximab preserved the binding ability of the antibody. Besides, the cytotoxic effect of 188Re-cetuximab was increased dose-dependently. NanoSPECT/CT imaging revealed that 188Re-cetuximab could continually target the tumor region for at least 48 h. Conclusion The highly specific targeted property of 188Re-cetuximab suggested that it is suitable as a diagnostic tool and maybe a potent radioimmunotherapy agent in EGFR-positive cancers. |
| Databáze: | OpenAIRE |
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