Inhalation of Ultrafine Particles Alters Blood Leukocyte Expression of Adhesion Molecules in Humans
| Autor: | Donna M. Speers, Li-Shan Huang, Judith C. Stewart, Christopher Cox, Lauren M. Frasier, David Chalupa, Mark J. Utell, Günter Oberdörster, Anthony P. Pietropaoli, Mark W. Frampton, Paul E. Morrow |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty Health Toxicology and Mutagenesis Lymphocyte CD18 010501 environmental sciences 01 natural sciences 03 medical and health sciences Antigens CD Internal medicine Ultrafine particle medicine Leukocytes Humans human Respiratory system Particle Size Exercise 030304 developmental biology 0105 earth and related environmental sciences Inhalation exposure 0303 health sciences Air Pollutants Inhalation Exposure Inhalation Cell adhesion molecule Chemistry Research Public Health Environmental and Occupational Health Dust Venous blood Asthma Carbon ultrafine particles Endocrinology medicine.anatomical_structure blood leukocytes 13. Climate action Immunology Female monocytes |
| Zdroj: | Environmental Health Perspectives |
| ISSN: | 1552-9924 0091-6765 |
| Popis: | Ultrafine particles (UFPs; aerodynamic diameter100 nm) may contribute to the respiratory and cardiovascular morbidity and mortality associated with particulate air pollution. We tested the hypothesis that inhalation of carbon UFPs has vascular effects in healthy and asthmatic subjects, detectable as alterations in blood leukocyte expression of adhesion molecules. Healthy subjects inhaled filtered air and freshly generated elemental carbon particles (count median diameter approximately 25nm, geometric standard deviation approximately 1.6), for 2 hr, in three separate protocols: 10 microg/m3 at rest, 10 and 25 microg/m3 with exercise, and 50 microg/m3 with exercise. In a fourth protocol, subjects with asthma inhaled air and 10 microg/m3 UFPs with exercise. Peripheral venous blood was obtained before and at intervals after exposure, and leukocyte expression of surface markers was quantitated using multiparameter flow cytometry. In healthy subjects, particle exposure with exercise reduced expression of adhesion molecules CD54 and CD18 on monocytes and CD18 and CD49d on granulocytes. There were also concentration-related reductions in blood monocytes, basophils, and eosinophils and increased lymphocyte expression of the activation marker CD25. In subjects with asthma, exposure with exercise to 10 microg/m3 UFPs reduced expression of CD11b on monocytes and eosinophils and CD54 on granulocytes. Particle exposure also reduced the percentage of CD4+ T cells, basophils, and eosinophils. Inhalation of elemental carbon UFPs alters peripheral blood leukocyte distribution and expression of adhesion molecules, in a pattern consistent with increased retention of leukocytes in the pulmonary vascular bed. |
| Databáze: | OpenAIRE |
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