Malformations persist after metamorphosis ofXenopus laevis tadpoles exposed to Ni2+, Co2+, or Cd2+ in FETAX assays
| Autor: | Sunderman Fw, Sidney M. Hopfer, Martin J, Grbac-Ivankovic S, Plowman Mc |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Time Factors
Health Toxicology and Mutagenesis media_common.quotation_subject Xenopus Ovary Toxicology Andrology Xenopus laevis Nickel Salientia Genetics medicine Animals Juvenile Metamorphosis malformations Genetics (clinical) media_common Larva biology Metamorphosis Biological Abnormalities Drug-Induced Cobalt Anatomy biology.organism_classification Teratology medicine.anatomical_structure Oncology Toxicity Cadmium |
| Zdroj: | Teratogenesis, Carcinogenesis, and Mutagenesis. 14:135-144 |
| ISSN: | 1520-6866 0270-3211 |
| DOI: | 10.1002/tcm.1770140305 |
| Popis: | This study was performed to determine whether malformations induced in Xenopus laevis embryos by exposures to divalent nickel, cobalt, or cadmium chlorides in FETAX assays persist after the tadpoles undergo metamorphosis to juvenile frogs. Embryos were exposed for four days to EC50 concentrations of Ni2+, Co2+, or Cd2+ under the standard conditions of FETAX assays ; thereafter, the exposures were discontinued and the tadpoles were kept in aquaria through metamorphosis. Controls were treated similarly, without exposure to metals. At 13 weeks of age, surviving frogs were killed and examined for malformations. Control and metal-exposed groups of Xenopus did not differ significantly in their median ages at metamorphosis, mean body weights, or survival at 13 weeks. Overall incidences of malformations found in Ni(2+)-, Co(2+)-, or Cd(2+)-exposed frogs at 13 weeks of age were 55, 40, and 51%, respectively (P < 0.01 vs. 3% in controls). The malformations of metal-exposed frogs included retinal depigmentation, diastematomyelia, scoliosis, kyphosis, phocomelia, sacro-pelvic and hind- limb deformities, and dysplasia of the heart, kidney, ovary and gut. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text