Sphingolipids control dermal fibroblast heterogeneity

Autor:	Laura Capolupo, Irina Khven, Alex R. Lederer, Luigi Mazzeo, Galina Glousker, Sylvia Ho, Francesco Russo, Jonathan Paz Montoya, Dhaka R. Bhandari, Andrew P. Bowman, Shane R. Ellis, Romain Guiet, Olivier Burri, Johanna Detzner, Johannes Muthing, Krisztian Homicsko, François Kuonen, Michel Gilliet, Bernhard Spengler, Ron M. A. Heeren, G. Paolo Dotto, Gioele La Manno, Giovanni D’Angelo
Přispěvatelé:	Imaging Mass Spectrometry (IMS), RS: M4I - Imaging Mass Spectrometry (IMS)
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Fibroblasts/metabolism EXPRESSION Sphingolipids Multidisciplinary IDENTIFICATION INHIBITION MASS-SPECTROMETRY ORGANIZATION Fibroblasts VARIABILITY REGENERATION TISSUE SINGLE-CELL ANALYSIS Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Lipidomics Sphingolipids/chemistry Humans lipids (amino acids peptides and proteins) Transcriptome Metabolic Networks and Pathways Skin LIPID EXTRACTION
Zdroj:	Science, 376(6590):eabh1623. American Association for the Advancement of Science
ISSN:	0036-8075
DOI:	10.1126/science.abh1623
Popis:	Human cells produce thousands of lipids that change during cell differentiation and can vary across individual cells of the same type. However, we are only starting to characterize the function of these cell-to-cell differences in lipid composition. Here, we measured the lipidomes and transcriptomes of individual human dermal fibroblasts by coupling high-resolution mass spectrometry imaging with single-cell transcriptomics. We found that the cell-to-cell variations of specific lipid metabolic pathways contribute to the establishment of cell states involved in the organization of skin architecture. Sphingolipid composition is shown to define fibroblast subpopulations, with sphingolipid metabolic rewiring driving cell-state transitions. Therefore, cell-to-cell lipid heterogeneity affects the determination of cell states, adding a new regulatory component to the self-organization of multicellular systems.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::110e0c2c2d1411b0740884ffa8b0473d https://doi.org/10.1126/science.abh1623 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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