Ameliorating Spinal Cord Injury in an Animal Model With Mechanical Tissue Resuscitation
| Autor: | Stephen B. Tatter, Maria P. McGee, Heather L. Green, Louis C. Argenta, Stan Gordon, Zhenlin Zheng, Michael J. Morykwas |
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| Rok vydání: | 2016 |
| Předmět: |
Resuscitation
Cord Swine Traumatic brain injury 030218 nuclear medicine & medical imaging Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Edema medicine Animals Spinal cord injury Spinal Cord Injuries medicine.diagnostic_test business.industry Magnetic resonance imaging Recovery of Function medicine.disease Spinal cord Magnetic Resonance Imaging Rats Disease Models Animal Diffusion Tensor Imaging medicine.anatomical_structure Spinal Cord Anesthesia Surgery Neurology (clinical) medicine.symptom business Negative-Pressure Wound Therapy 030217 neurology & neurosurgery Diffusion MRI |
| Zdroj: | Neurosurgery. 78:868-876 |
| ISSN: | 0148-396X |
| Popis: | Background Traumatic spinal cord injury (SCI) is a major worldwide cause of mortality and disability with limited treatment options. Previous research applying controlled negative pressure to traumatic brain injury in rat and swine models resulted in smaller injuries and more rapid recovery. Objective To examine the effects of the application of a controlled vacuum (mechanical tissue resuscitation [MTR]) to SCI in a rat model under several magnitudes of vacuum. Methods Controlled contusion SCIs were created in rats. Vacuums of -50 and -75 mm Hg were compared. Analysis included open-field locomotor performance, magnetic resonance imaging (in vivo T2, ex vivo diffusion tensor imaging and fiber tractography), and histological assessments. Results MTR treatment significantly improved the locomotor recovery from a Basso, Beattie, and Bresnahan score of 7.8 ± 1.9 to 11.4 ± 1.2 and 10.7 ± 1.9 at -50- and -75-mm Hg pressures, respectively, 4 weeks after injury. Both pressures also reduced fluid accumulations > 10% by T2-imaging in SCI sites. The mean fiber number and mean fiber length were greater across injured sites after MTR treatment, especially with treatment with -50 mm Hg. Myelin volume was increased significantly by 60% in the group treated with -50 mm Hg. Conclusion MTR of SCI in a rat model is effective in reducing edema in the injured cord, preserving myelin survival, and improving the rate and quantity of functional recovery. Abbreviations BBB, Basso, Beattie, and BresnahanDTI, diffusion tensor imagingFA, fractional anisotropyMTR, mechanical tissue resuscitationMTR50, mechanical tissue resuscitation with 50-mm Hg subatmospheric pressureMTR75, mechanical tissue resuscitation with 75-mm Hg subatmospheric pressureROI, region of interestSCI, spinal cord injury. |
| Databáze: | OpenAIRE |
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