Determination and stability of some international screening limited drugs in equine blood by LC-MS/MS
| Autor: | Erol Kabil, Serol Korkmaz, Eylem Funda Göktaş, Tuba Asena Kadioğlu, Aysen Kurt Cucu, Ertuğrul Güneş |
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| Přispěvatelé: | Goktas, Eylem Funda, Kabil, Erol, Gunes, Ertugrul, Korkmaz, Serol, Kadioglu, Tuba Asena, Cucu, Aysen Kurt |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Stability test
TERM STABILITY STORAGE-CONDITIONS 01 natural sciences Matrix (chemical analysis) Hydrolysis TESTOSTERONE Lc ms ms Veteriner Hekimlik LC-MS/MS Doping control equine blood LC-MS/MS stability equine blood Whole blood Wax Chromatography General Veterinary Chemistry Doping control stability URINE SAMPLES 0104 chemical sciences 010404 medicinal & biomolecular chemistry Veterinary visual_art visual_art.visual_art_medium DOPING AGENTS Animal Science and Zoology |
| Zdroj: | Volume: 66, Issue: 4 363-372 Ankara Üniversitesi Veteriner Fakültesi Dergisi |
| ISSN: | 1308-2817 |
| Popis: | Stability of some international screening limited therapeutic substances for doping control in equine blood has been investigated with a validated method by using LC-MS/MS after chemical hydrolysis with orthophosphoric acid. Whole blood samples were extracted with a WAX cartridge on auto-SPE. Stability test of the drug substances performed at the same time in working solution and matrix at +4 degrees C, -20 degrees C, +20 degrees C in dark and +20 degrees C in light conditions for 1, 2, 3 and 4 weeks. For short-time stability, the effects of 6 h storage at +55 degrees C was also investigated. Repeated data were statistically analyzed with ANOVA. All substances in working solution were stable at -20 degrees C and +4 degrees C. In the blood matrix, higher temperatures (+20 degrees C) in light and dark caused degradation of substances at the end of four weeks and short-term study (P |
| Databáze: | OpenAIRE |
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