Expression of TAR RNA-Binding Protein in Baculovirus and Co-Immunoprecipitation with Insect Cell Protein Kinase
| Autor: | B. Wendy Snowden, Anne Gatignol, Monsef Benkirane, Christopher M. Roberts, Kuan-Teh Jeang, Edward D. Blair |
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| Rok vydání: | 1995 |
| Předmět: |
Kinase
Immunoprecipitation viruses Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry RNA-binding protein Sf9 Cell Biology General Medicine Biology Protein kinase R Molecular biology Cell biology Complementary DNA Pharmacology (medical) Protein kinase A Molecular Biology Nuclear localization sequence |
| Zdroj: | Journal of Biomedical Science. 2:322-329 |
| ISSN: | 1423-0127 1021-7770 |
| Popis: | TAR RNA-binding protein TRBP was originally isolated by its binding affinity for radiolabeled HIV-1 leader RNA. Subsequent studies have suggested that this protein is one member of a family of double-stranded RNA-binding proteins. Recent findings indicate that TRBP might function to antagonize the translational inhibitory effect that can be mediated through cellular protein kinase, PKR. Here, we report on the over-expression of a cDNA coding for TRBP in eukaryotic SF9 cells using baculovirus. We characterized the nuclear localization of TRBP in insect cells, and we demonstrate that TRBP co-immunoprecipitates with a protein in these cells antigenically related to human PKR. Copyright 1995 S. Karger AG, Basel |
| Databáze: | OpenAIRE |
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