Formation and Evaluation of a Two-Phase Polymer System in Human Plasma as a Method for Extracellular Nanovesicle Isolation
| Autor: | Anastasiia Merdalimova, Anastasia Malek, Vladimir V. Egorov, Inga Nazarova, Natalia Yevlampieva, Maria Slyusarenko, Yuri P. Garmay, Nadezhda Nikiforova, Dmitry A. Gorin, Elena Sidina |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Polymers and Plastics
Nanoparticle tracking analysis diagnostic 02 engineering and technology Polyethylene glycol Article Flow cytometry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound lcsh:Organic chemistry medicine Extracellular plasma 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography medicine.diagnostic_test Molecular mass Chemistry Vesicle General Chemistry Polymer 021001 nanoscience & nanotechnology dextran polyethylene glycol extracellular nanovesicles Polymer blend 0210 nano-technology two-phase polymer system |
| Zdroj: | Polymers, Vol 13, Iss 458, p 458 (2021) Polymers Volume 13 Issue 3 |
| ISSN: | 2073-4360 |
| Popis: | The aim of the study was to explore the polyethylene glycol–dextran two-phase polymer system formed in human plasma to isolate the exosome-enriched fraction of plasma extracellular nanovesicles (ENVs). Systematic analysis was performed to determine the optimal combination of the polymer mixture parameters (molecular mass and concentration) that resulted in phase separation. The separated phases were analyzed by nanoparticle tracking analysis and Raman spectroscopy. The isolated vesicles were characterized by atomic force microscopy and dot blotting. In conclusion, the protein and microRNA contents of the isolated ENVs were assayed by flow cytometry and by reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR), respectively. The presented results revealed the applicability of a new method for plasma ENV isolation and further analysis with a diagnostic purpose. |
| Databáze: | OpenAIRE |
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