| Popis: |
Background: Chitin is an organic polymer and it is rich marine natural polysaccharide after the cellulose. The main natural sources of chitin are exoskeletons of insects, mollusks, the cell walls of certain fungi and crustaceans such as crabs, shrimps and lobsters. The waste of these marine exoskeletons are pollutant for the environment continuously, but this raw material could be used for the production of commercial chitin. The chitin is an important raw material used for water treatment, agricultural, biomedicinal, biotechnological purposes, food and paper industry and cosmetics. Based on the variety of importance, the present targets of this study is to i. optimize the demineralization process for the removal of calcium and phosphorus from the waste of Portunidae segnis (P. segnis) by using acid at ambient temperature ii. characterize the isolated demineralized sample as well as the percentage of remaining calcium and phosphorus by using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). Results: The coarse powder samples of P. segnis were demineralized with seven different concentrations of hydrochloric acid at ambient temperature for 1 hour. All the demineralization samples at different concentrations were analysed by using sensitive ICP-OES. The results based on ICP-OES showed that among the seven different concentrations wused in the demineralization process for the isolation of chitin, the best was 2M of HCl concentration for the production of chitin. Conclusion: The develop optimized demineralization process could be used commercially for the isolation of chitin for the preparation of agriculture, biomedicine, biotechnological purposes. |
