The effect of a probiotic Escherichia coli strain on regulatory T-cells in six year-old children
| Autor: | Libuše Kolářová, Jiří Hrdý, Raja Lodinová-Žádníková, L. Prokešová, I Kocourková |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Microbiology (medical) Allergy medicine.medical_treatment chemical and pharmacologic phenomena Biology T-Lymphocytes Regulatory Microbiology law.invention Flow cytometry 03 medical and health sciences Probiotic 0302 clinical medicine Immune system law Escherichia coli Hypersensitivity medicine Humans Child medicine.diagnostic_test Probiotics Incidence (epidemiology) Infant Newborn Infant FOXP3 Interleukin Flow Cytometry medicine.disease Interleukin-10 030104 developmental biology Cytokine 030228 respiratory system Child Preschool Immunology Cytokines Female |
| Zdroj: | Beneficial Microbes. 7:639-648 |
| ISSN: | 1876-2891 1876-2883 |
| Popis: | Probiotics are believed to prevent or reduce allergy development but the mechanism of their beneficial effect is still poorly understood. Immune characteristics of regulatory T cells (Tregs) in peripheral blood of perinatally probiotic-supplemented children of allergic mothers (51 children), non-supplemented children of allergic mothers (42 children), and non-supplemented children of healthy mothers (28 children) were compared at the age of 6-7 years. A first dose of a probiotic Escherichia coli strain (E. coli O83:K24:H31) was administered within 2 days after the birth and then 12 times during the first months of life and children were followed longitudinally. Proportion and functional properties of Tregs were estimated by flow cytometry in relation to the children’s allergy status. Proportion of Tregs in the peripheral blood of children suffering from allergy tends to be higher whereas median of fluorescence intensity (MFI) of FoxP3 was significantly decreased in allergic group. Intracellular presence of regulatory cytokine interleukin (IL)-10 was also lower in allergic children. Immune functions of Tregs reflected by both MFI of FoxP3 and IL-10 in the group of probiotic-supplemented children of allergic mothers were nearly comparable with children of healthy mothers while probiotic non-supplemented children of allergic mothers have decreased immune function of Tregs. Supplementation by probiotic E. coli strain decreases allergy incidence in high-risk children. In contrast to our expectation, proportion of Tregs has not been increased in probiotic supplemented children. Beneficial effect of probiotics on newborn immature immune system could be, at least partially, explained by the modulating immune function of Tregs. In summary, we detected increased proportion of Tregs in peripheral blood of allergic children, their functional properties were decreased in comparison with the Tregs of healthy children. A unifying hypothesis for these findings is that Treg numbers in allergic children are increased in order to compensate for decreased function. |
| Databáze: | OpenAIRE |
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