A signal-on electrochemical aptasensor based on silanized cellulose nanofibers for rapid point-of-use detection of ochratoxin A
| Autor: | Nitin Nitin, Ahmed Y. El-Moghazy, Noha Amaly, Gang Sun, Georges Istamboulie |
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| Rok vydání: | 2020 |
| Předmět: |
Working electrode
Aptamer Immobilized Nucleic Acids Nanofibers Bioengineering Food Contamination Coffee analysis 02 engineering and technology Biosensing Techniques Aptamers 01 natural sciences Coffee Article Analytical Chemistry chemistry.chemical_compound Limit of Detection Nanotechnology Cellulose Cellulose nanofibers Propylamines 010401 analytical chemistry Electrochemical aptasensor Ochratoxin A Electrochemical Techniques Aptamers Nucleotide Silanes 021001 nanoscience & nanotechnology Cellulose acetate Ochratoxins 0104 chemical sciences Methylene Blue Point-of-use Membrane chemistry Chemical engineering Silanization Nanofiber Triethoxysilane Other Chemical Sciences 0210 nano-technology Nucleotide Oxidation-Reduction |
| Zdroj: | Mikrochim Acta Mikrochimica acta, vol 187, iss 9 |
| ISSN: | 1436-5073 |
| Popis: | An innovative ultrasensitive electrochemical aptamer-based sensor was developed for ochratoxin A (OTA) detection in cold brew coffee through revolutionary combination of nanofibers, electrochemicalmethod, and aptamer technologies. The assembly of the aptasensor was based on the activation of silanized cellulose nanofibrous membranes as a supporting matrix for methylene blue (MB) redox probe-labeled aptamer tethering. Cellulose nanofibrous membranes were regenerated by deacetylating electrospun cellulose acetate nanofibrous membranes with deacetylation efficacy of 97%, followed by silanization of the nanofiber surfaces by using (3-aminopropyl)triethoxysilane (APTES). A replacement of conventionally casted membranes by the nanofibrous membranes increased the active surface area on the working electrode of a screen-printed three-electrode sensor by more than two times, consequently enhancing the fabricated aptasensor performance. The developed aptasensor demonstrated high sensitivity and specificity toward OTA in a range 0.002-2ngmL-1, with a detection limit of 0.81pgmL-1. Moreover, the assembled aptamer-based sensor successfully detected OTA in cold brew coffee samples without any pretreatment. The aptasensor exhibited good reusability and stability over longstorage time. Graphical abstract. |
| Databáze: | OpenAIRE |
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