Low intensity lasers differently induce primary human osteoblast proliferation and differentiation
| Autor: | Aline de Lima Leite, Cintia Kazuko Tokuhara, Rodrigo Cardoso de Oliveira, Maria Aparecida de Andrade Moreira Machado, Adriana Arruda Matos, V. S. Bagnato, Mariana R. Santesso, Camila Peres-Buzalaf, Flávia Amadeu de Oliveira |
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| Rok vydání: | 2016 |
| Předmět: |
MAPK/ERK pathway
Cell Survival MAP Kinase Signaling System Cellular differentiation Biophysics Collagen Type I OSTEOBLASTO 030207 dermatology & venereal diseases 03 medical and health sciences Calcification Physiologic 0302 clinical medicine Downregulation and upregulation Osteogenesis Gene expression medicine Humans Osteonectin Radiology Nuclear Medicine and imaging Low-Level Light Therapy Cell Proliferation Osteoblasts Radiation Radiological and Ultrasound Technology biology Cell growth Chemistry Cell Differentiation Osteoblast 030206 dentistry Cell biology medicine.anatomical_structure Immunology biology.protein Biomarkers Type I collagen |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Among various compounds used in research and clinic for degenerative bone diseases, low level laser therapy (LLLT), comprising low level lasers (LLL) and light emitting diodes (LEDs), has been investigated regarding its effects on bone metabolism. They have specific wavelengths but in general act as a cellular biomodulator, and as a therapeutic agent, rebalancing and normalizing their activity. However, they are not standardized yet, since their parameters of use are relevant for the effects and mechanisms of action. Therefore, the aim of this study was to compare the influence of two spectrums of LLL and LED phototherapy, at the same energy densities (10 and 50J/cm(2)), on human osteoblasts proliferation and differentiation. The involvement of ERK signaling on proliferation was also investigated by evaluating its activation during proliferation under different phototherapies by western blotting and CFSE-based osteoblast proliferation was measured in a presence or absence of the ERK-specific inhibitor. Osteogenic differentiation was evaluated through in vitro mineralization and gene expression of type I collagen (COL1A1) and osteonectin (SPARC) by Real Time- PCR. Increases in viable cells and proliferation were obtained after irradiation, regardless of LLLT type. However, only red at 10J/cm(2) and infrared at both doses, but not LED, induced ERK1/2 activation. In the presence of ERK inhibitor, the LLL-induced proliferation was prevented. In addition, while COL1A1 gene expression was upregulated by red laser, SPARC does so by infrared stimulation. However, LED, at both doses, increased both COL1A1 and SPARC expression. All LLLT increased mineralization, dependent on the dose and time. Thus, LLL and LED differently modulated the metabolism of human osteoblasts, increasing proliferation by mechanism dependent or not of ERK signaling activation and osteogenic differentiation markers. |
| Databáze: | OpenAIRE |
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