Flavin fluorescence lifetime imaging of living peripheral blood mononuclear cells on micro and nano-structured surfaces
| Autor: | A. Marcek Chorvatova, Dusan Chorvat, Anton Mateasik, J. Bruncko, Tibor Teplicky, C. Gladine, J. Horilova, Ingrid Lajdova |
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| Přispěvatelé: | International Laser Centre, Unité de Nutrition Humaine (UNH), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Slovak Medical University of Bratislava (SMU), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université |
| Rok vydání: | 2015 |
| Předmět: |
FLIM
Fluorescence-lifetime imaging microscopy Materials science Flavoprotein Nanotechnology 02 engineering and technology Flavin group ZnO nanosurfaces confocal microscopy 01 natural sciences OrmoComp polymers Pulsed laser deposition law.invention 010309 optics Confocal microscopy law 0103 physical sciences microfabrication human peripheral blood mononuclear cells biology technology industry and agriculture 021001 nanoscience & nanotechnology Fluorescence zno biology.protein Biophysics 2-photon polymerisation 0210 nano-technology Luminescence [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Layer (electronics) |
| Zdroj: | Proceedings of SPIE-International Society for Optical Engineering Multiphoton Microscopy in the Biomedical Sciences XV Multiphoton Microscopy in the Biomedical Sciences XV, Feb 2015, San Francisco, United States. 334 p., ⟨10.1117/12.2076706⟩ |
| ISSN: | 0277-786X |
| Popis: | International audience; Fabricated micro-and nano-structured surfaces were evaluated for use with living cells. Metabolic state was tested by means of endogenous flavin fluorescence of living peripheral blood mononuclear cells (PBMC) positioned on a coverslip, non-covered, or covered with micro-or nano-structured surfaces (OrmoComp polymer structures produced by 2-photon photopolymerisation, or Zinc Oxide (ZnO) layer fabricated by pulsed laser deposition). Confocal microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) were employed to gather flavin fluorescence lifetime images of living PBMC on structured surfaces. Gathered data are the first step towards monitoring of the live cell interaction with different micro/nano-structured surfaces and thus evaluate their potential applicability in the biomedical field. |
| Databáze: | OpenAIRE |
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