Preferential adsorption of fetal bovine serum on bare and aromatic thiol-functionalized gold surfaces in cell culture media
| Autor: | Nam Woong Song, Jin-Ho Park, Jin Park, Erdene-Ochir Ganbold, Kwang-Su Ock, So Yeong Lee, Keunchang Cho, Sang-Woo Joo |
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| Rok vydání: | 2011 |
| Předmět: |
Surface Properties
Analytical chemistry Metal Nanoparticles Hydrocarbons Aromatic Biomaterials Tissue Culture Techniques Colloid and Surface Chemistry Adsorption Animals Sulfhydryl Compounds chemistry.chemical_classification Quartz crystal microbalance Fetal Blood Blood proteins Surfaces Coatings and Films Electronic Optical and Magnetic Materials Culture Media chemistry Transmission electron microscopy Colloidal gold Thiol Cattle Gold Intracellular Fetal bovine serum Nuclear chemistry |
| Zdroj: | Journal of colloid and interface science. 363(1) |
| ISSN: | 1095-7103 |
| Popis: | Intracellular uptake of serum-coated gold nanoparticles (AuNPs) in a single mammalian cell was examined in order to investigate the interactions of cell culture media and aromatic thiol-functionalized gold surfaces using micro-spectroscopic tools. The AuNPs modified by the aromatic thiols of para-aminobenzenethiol (ABT), para-hydroxy benzenethiol (HBT), and para-carboxylic benzenethiol (CBT, para-mercaptobenzoic acid) bearing NH(2), OH, and COOH surface functional groups are presumed to adsorb the serum proteins as indicated from the compiled quartz crystal microbalance (QCM) data. The QCM results indicate that among the constituents, fetal bovine serum (FBS) should be the major adsorbate species on AuNPs incubated in Roswell Park Memorial Institute (RPMI) medium. The functionalized AuNPs were found to be internalized as an aggregation state in mammalian cells as evidenced by transmission electron microscopy (TEM) images. We monitored such cellular uptake behaviors of aromatic thiol-modified AuNPs using dark-field microscopy (DFM)-guided confocal surface-enhanced Raman scattering techniques in order to identify the three-dimensional localization inside the single cell. We found that the uptake amounts of ABT, HBT, and CBT were similar by counting up to 70 particles inside the cells incubated in the solution mixture of the aromatic thiol and 1,4-phenylenediisocyanide (PDIC) as a reference. This result indicates for the short aromatic thiol compounds, the AuNPs should enter the cell after the serum-coating regardless of the surface functional groups. Considering that the aromatic thiols have little effect on the serum coating, the DFM/SERS method is an effective tool for monitoring the localization of AuNPs inside a single cell. |
| Databáze: | OpenAIRE |
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