Enantioselective determination of bromoisovalerylurea by liquid chromatography on chiral stationary phase in reversed- or normal-phase partition mode
| Autor: | Rumiko Kondo, Yoshito Kamijo, Yoshio Ohtani, Hisako Takeuchi, Takanori Okuda, Hideki Ohtani, Takashi Nishikawa |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Saliva Clinical Biochemistry Biochemistry Analytical Chemistry chemistry.chemical_compound Phase (matter) Drug Discovery Humans Hypnotics and Sedatives Partition (number theory) Molecular Biology Chromatography High Pressure Liquid Pharmacology Chromatography Chemistry Extraction (chemistry) Enantioselective synthesis Stereoisomerism General Medicine Chiral column chromatography Calibration Urea Spectrophotometry Ultraviolet Bromisovalum Drug Overdose Enantiomer |
| Zdroj: | Biomedical Chromatography. 14:243-248 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/1099-0801(200006)14:4<243::aid-bmc977>3.0.co;2-i |
| Popis: | Bromoisovalerylurea (bromvalerylurea) is a sedative-hypnotic given orally as a racemate. Enantiomers of this drug could be separated by high-performance liquid chromatography on the three chiral stationary phases (a vancomycin-bonded, beta-cyclodextrin derivative-bonded, or urea derivative-bonded phase). Biological fluids of human subjects who had ingested toxic or therapeutic doses of the racemate were chromatographed after liquid-liquid extraction. The (+)-enantiomer concentration was almost equal to the (-)-enantiomer concentration in the serum of one overdosed patient. In all the other subjects, the (+)-enantiomer was less than the (-)-enantiomer in their sera and saliva. The data suggest that the drug is absorbed non-stereoselectively from the gastrointestinal tract and eliminated from the blood stereoselectively. |
| Databáze: | OpenAIRE |
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