Influences of intrauterine semen administration on regulatory T lymphocytes in the oestrous mare (Equus caballus)
| Autor: | Christine Aurich, Constanze Hartmann, Wilhelm Gerner, Ingrid Walter, Armin Saalmüller |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
endocrine system Uterus chemical and pharmacologic phenomena Semen Endometrium Insemination T-Lymphocytes Regulatory Andrology 03 medical and health sciences Immune system Food Animals Estrus Pregnancy medicine Immune Tolerance Animals Horses Lymphocyte Count Small Animals reproductive and urinary physiology Insemination Artificial Estrous cycle urogenital system Equine business.industry FOXP3 Horse hemic and immune systems Forkhead Transcription Factors Abortion Veterinary Immunohistochemistry 030104 developmental biology medicine.anatomical_structure Animal Science and Zoology Female business |
| Zdroj: | Theriogenology. 118 |
| ISSN: | 1879-3231 |
| Popis: | In the mare, early pregnancy loss is common, but involvement of the maternal immune system in the pathogenesis of this condition has not been investigated in detail so far. In the present study, we assessed effects of exposure of the endometrium to semen or seminal plasma in oestrous mares on the response of regulatory T lymphocytes (Tregs) in the peripheral circulation as well as in the endometrium. Raw semen, seminal plasma or PBS (control) were introduced into the uterus of oestrous mares (n = 12). Blood was collected immediately before insemination or PBS infusion (time 0), and 12, 24 and 48 h thereafter. Endometrial biopsies were collected at 24 h. In peripheral blood, Treg (CD4+Foxp3+) cells were determined by flow cytometry. In endometrial biopsies, Tregs were assessed as cells staining positive for Foxp3 by immunohistochemistry. The percentage of Tregs in blood decreased (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect