Enhanced inhibition of estrogen receptor nuclear binding in the uterus of aged mice
| Autor: | Diego Bellabarba, Jean-Guy Lehoux, Serge Belisle |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Aging
medicine.medical_specialty medicine.drug_class Ratón Ovariectomy Uterus Estrogen receptor Biology Biochemistry Mice Cytosol Endocrinology Internal medicine medicine Animals Cell Nucleus Estradiol DNA Mice Inbred C57BL Cell nucleus medicine.anatomical_structure Receptors Estrogen Estrogen In utero Ovariectomized rat Female |
| Zdroj: | Journal of Steroid Biochemistry. 26:521-526 |
| ISSN: | 0022-4731 |
| DOI: | 10.1016/0022-4731(87)90002-1 |
| Popis: | We have studied why uteri from aging mice show a decrease nuclear concentration of estrogen receptors (UER). While 50-60% of available cytosolic UER from ovariectomized (OVX) mice aged 4-8 months, upon physiochemical activation, are able to bind either to DNA-cellulose or nuclear suspensions from young animals, only 20-30% of comparable concentrations of cytosolic UER from mice aged 15-18 months did so under identical experimental conditions. Nuclear dilutions with uterine cytosolic fractions from estrogen treated OVX mice prior to determination of [3H] UER binding sites in nuclear suspensions decreased the number of nuclear ER sites in both age groups. However, we observed that cytosols from aged animals showed a greater ability to prevent [3H]E2 binding to nuclear sites when compared to young ones (inhibition index: 0.286 +/- 0.013 (SE) vs. 0.137 +/- 0.025, P less than 0.05). These changes occur independently of protein concentration and result from dilution of a specific endogenous inhibitor of [3H]E2 binding to nuclear sites. The significance of these observed differences is discussed. |
| Databáze: | OpenAIRE |
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