Characterization of intra-graft B cells during renal allograft rejection
| Autor: | Valeriya Zarkhin, Li Li, Shirley Kwok, Minnie M. Sarwal, Oscar Salvatierra, Neeraja Kambham, Szu-Chuan Hsieh |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Graft Rejection Male Pathology medicine.medical_specialty Plasma Cells graft survival B-Lymphocyte Subsets CD38 renal allograft rejection Immunophenotyping Antigen Biopsy B-lymphocytes medicine Complement C4b Humans CD20 Kidney Membrane Glycoproteins biology medicine.diagnostic_test business.industry Middle Aged Antigens CD20 ADP-ribosyl Cyclase 1 Kidney Transplantation Peptide Fragments Transplantation medicine.anatomical_structure Nephrology Immunology biology.protein Female Antibody Chemokines transplant biopsy business intra-graft CD20+ and CD38+ infiltrates |
| Zdroj: | Kidney international. 74(5) |
| ISSN: | 1523-1755 |
| Popis: | Intra-graft CD20 + B-cell clusters are found during acute rejection of renal allografts and correlate with graft recovery following rejection injury. Here using archived kidney tissue we conducted immunohistochemical studies to measure specific subsets of pathogenic B cells during graft rejection. Cluster-forming CD20 + B cells in the rejected graft are likely derived from the recipient and are composed of mature B cells. These cells are activated (CD79a + ), and present MHC Class II antigen (HLADR + ) to CD4 + T cells. Some of these clusters contained memory B cells (CD27 + ) and they did not correlate with intra-graft C4d deposition or with detection of donor-specific antibody. Further, several non-cluster forming CD20 − B-lineage CD38 + plasmablasts and plasma cells were found to infiltrate the rejected grafts and these cells strongly correlated with circulating donor-specific antibody, and to a lesser extent with intra-graft C4d. Both CD20 + B cells and CD38 + cells correlated with poor response of the rejection to steroids. Reduced graft survival was associated with the presence of CD20 cells in the graft. In conclusion, a specific subset of early lineage B cells appears to be an antigen-presenting cell and which when present in the rejected graft may support a steroid-resistant T-cell-mediated cellular rejection. Late lineage interstitial plasmablasts and plasma cells may also support humoral rejection. These studies suggest that detailed analysis of interstitial cellular infiltrates may allow better use of B-cell lineage specific treatments to improve graft outcomes. |
| Databáze: | OpenAIRE |
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