Maternal and Neonatal Factors Associated with the High Yield of Mononuclear Low-Density/CD34+ Cells from Placental/Umbilical Cord Blood
| Autor: | Haruo Sato, Atsuko Omori, Hisateru Ohba, Ikuo Kashiwakura, Kenji Takahashi, Kaori Kudo, Masaharu Hazawa, Tsuneo A. Takahashi, Naoko Misaki, Mami Manabe |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
medicine.medical_specialty Amniotic fluid Adolescent CD34 Antigens CD34 Umbilical cord General Biochemistry Genetics and Molecular Biology Andrology Leukocyte Count Meconium medicine Humans Progenitor cell Obstetrics business.industry Infant Newborn Gestational age General Medicine Middle Aged Fetal Blood Hematopoietic Stem Cells Blood Cell Count Haematopoiesis medicine.anatomical_structure Cord blood Female business |
| Zdroj: | The Tohoku Journal of Experimental Medicine. 215:23-32 |
| ISSN: | 1349-3329 0040-8727 |
| DOI: | 10.1620/tjem.215.23 |
| Popis: | Placental/umbilical cord blood (CB) contains nucleated cells and hematopoietic stem/progenitor cells (CD34(+) cells). However it is difficult to predict the number of nucleated/CD34(+) cells in each CB before cell processing. Despite many previous studies from institutes affiliated with CB banks in metropolitan areas, little information is available regarding the characteristics of CB units from other medical facilities. The purpose of the present study was to analyze the maternal/neonatal factors on the yield of cells in CB units. A total of 176 CB units were obtained from single-birth and normal vaginal deliveries. Mononuclear low-density (LD) cells were separated using Ficoll-Paque within 24 hrs after CB collection and then processed for the purification of CD34(+) cells. A multiple linear regression analysis was performed to assess the correlations between the yield of cells and maternal/neonatal factors including maternal age, gravid status, duration of labor, gestational age, neonatal height and weight, cord length, and meconium in the amniotic fluid. The total LD cells per CB unit had a weak positive correlation with the maternal age of primigravidae. The total LD cells per CB unit from the primigravidae agedor = 25 were significantly higher than those from the primigravidae agedor = 24. The total CD34(+) cells per CB unit from the 1-gravidae were significantly higher than those from the 2-gravidae and 3-gravidae, respectively among all donors. These results indicate that the CB units from the primigravidae agedor = 25 are more likely to contain higher yield of LD/CD34(+) cells. |
| Databáze: | OpenAIRE |
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