Upconversion nanoparticles-based FRET system for sensitive detection of Staphylococcus aureus
| Autor: | Huanhuan Li, Yongcun Yang, Qin Ouyang, Shujat Ali, Li Wang, Quansheng Chen |
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| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Aptamer Metal Nanoparticles 02 engineering and technology Biosensing Techniques 010402 general chemistry medicine.disease_cause 01 natural sciences Analytical Chemistry Limit of Detection medicine Fluorescence Resonance Energy Transfer Instrumentation Spectroscopy Detection limit Chromatography Chemistry Aptamers Nucleotide 021001 nanoscience & nanotechnology Fluorescence Atomic and Molecular Physics and Optics Photon upconversion 0104 chemical sciences Förster resonance energy transfer Colloidal gold Gold 0210 nano-technology Biosensor |
| Zdroj: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 255 |
| ISSN: | 1873-3557 |
| Popis: | Staphylococcus aureus (S. aureus) is a pathogenic bacterium that seriously endangers food safety. Herein, a rapid, sensitive and specific aptasensor based on upconversion fluorescence resonance energy transfer (FRET) was developed for S. aureus detection in food. Aptamer-functionalized gold nanoparticles (AuNPs-aptamers) were bonded to cDNA-modified upconversion nanoparticles (UCNPs-cDNA) by complementary pairing, resulting in fluorescence quenching. After adding S. aureus into the system, the aptamers preferentially combined with S. aureus, dissociated UCNPs-cDNA from AuNPs-aptamers, and the fluorescence was recovered. Under optimized conditions, there was a significant linear correlation between fluorescence intensity and S. aureus concentration over the range 47–4.7 × 107 CFU/mL (R2 = 0.9904) with a detection limit of 10.7 CFU/mL. Furthermore, the precision and accuracy of the developed biosensor were validated using standard plate count method, yielding no significant differences. The proposed method has potential application for rapid and sensitive quantification of S. aureus in food. |
| Databáze: | OpenAIRE |
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