Inhibition of leukotriene synthesis by honokiol in rat basophilic leukemia cells
| Autor: | Sumio Miyazaki, Shuichi Yamamoto, Hakaru Tasaki, Rika Sato, Ikuko Kobayashi, Masafumi Zaitu, Muneaki Matsuo, Tomohiro Ichimaru, Eriko Muro, Shafigeh Miyanji, Yuhei Hamasaki |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Honokiol
Leukotrienes Leukotriene B4 Immunology Lignans Leukotriene-A4 hydrolase chemistry.chemical_compound Phospholipase A2 Anti-Allergic Agents Tumor Cells Cultured Immunology and Allergy Animals Leukotriene biology Leukotriene C4 Biphenyl Compounds hemic and immune systems General Medicine respiratory system Rats Biphenyl compound chemistry Leukemia Basophilic Acute Arachidonate 5-lipoxygenase biology.protein Leukotriene Antagonists lipids (amino acids peptides and proteins) |
| Zdroj: | International archives of allergy and immunology. 110(3) |
| ISSN: | 1018-2438 |
| Popis: | The effects of honokiol, a diphenyl compound extracted from a Chinese herbal medicine, on leukotriene (LT) synthesis were evaluated in rat basophilic leukemia (RBL) cells. The production of LTC4 and LTB4 stimulated by the Ca2+ ionophore A23187 was measured in RBL-1 cells by high-performance liquid chromatography. Honokiol inhibited the production of LTC4 and LTB4 stimulated by A23187 in RBL-1 cells. Honokiol did not inhibit either phospholipase A2 activity, measured by the release of 3H-arachidonic acid (AA), or LTC4 synthase and LTA4 hydrolase activities, measured with LTA4-free acid as substrate. The synthesis of LTC4 and LTB4 from AA in RBL-1 cell lysates in the presence of Ca2+ was inhibited by honokiol. These results indicate that honokiol blocks LT synthesis by inhibiting 5-lipoxygenase activity. Honokiol also inhibited immunoglobulin E-mediated production of these LTs in RBL-2H3 cells, which was measured by a specific radioimmunoassay (RIA). These results suggest that honokiol may exhibit antiallergic actions by inhibiting LT synthesis in immediate-type hyperreactivity. |
| Databáze: | OpenAIRE |
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