Cytotoxic T lymphocytes from humans with adenocarcinomas stimulated by native MUC1 mucin and a mucin peptide mutated at a glycosylation site
| Autor: | Nichol Dolby, Ji Yuan Wu, Ramila Philip, Karen E. Lowe, Lydia Kilinski, Stephen E. Wright, Sohel Talib, Jane S. Lebkowski, Janet S. Burnside, Kenneth E. Dombrowski |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Interleukin 2
Cancer Research Glycosylation medicine.medical_treatment Immunology Molecular Sequence Data Breast Neoplasms Biology Adenocarcinoma Peripheral blood mononuclear cell Natural killer cell medicine Tumor Cells Cultured Immunology and Allergy Cytotoxic T cell Humans Amino Acid Sequence Lymph node Pharmacology Ovarian Neoplasms Binding Sites Mucin Mucin-1 T lymphocyte Molecular biology digestive system diseases Peptide Fragments medicine.anatomical_structure Cytokine Mutagenesis Leukocytes Mononuclear Female K562 Cells medicine.drug T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of immunotherapy (Hagerstown, Md. : 1997). 23(1) |
| ISSN: | 1524-9557 |
| Popis: | MUC1 mucin peptides stimulated cytotoxic T lymphocytes (CTL) from humans with adenocarcinomas. Peripheral blood mononuclear cells, tumor-draining lymph node cells, or tumor-infiltrating lymphocytes were stimulated using mono-nuclear cells from humans with adenocarcinomas of breast or ovary, respectively, using (a) a native MUC1 mucin tandem repeat peptide of 20 amino acids (MUC1-mtr1) plus recombinant human interleukin-2 (IL-2), (b) the mutated (T3N) MUC1-mtr1 plus IL-2, or (c) immobilized anti-CD3 plus IL-2, or (d) IL-2 alone. The CTL stimulated by each of these four conditions were predominately CD4+. However, the CTL stimulated by either the native MUC1-mtr1 or (T3N) MUC1-mtr1 showed 5-10 times greater cytotoxicity of a breast cancer cell line that expresses MUC1 compared to CTL stimulated by either anti-CD3 + IL-2 or IL-2 alone. Each incubation condition generated CTL with different variable beta gene families of T-cell receptors, implying an oligoclonal expansion of a limited CTL repertoire for each. Thus, peptide-stimulated T cells showed expression of cytotoxic cells, which was not induced by nonspecific (anti-CD3 or IL-2) stimulation. |
| Databáze: | OpenAIRE |
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