Clickable tyrosine binding bifunctional linkers for preparation of DNA-protein conjugates
| Autor: | Antonina Vigovskaya, Ishtiaq Ahmed, Ljiljana Fruk, Dennis M. Bauer |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Streptavidin
Biomedical Engineering Pharmaceutical Science DNA Single-Stranded Bioengineering 010402 general chemistry 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Complementary DNA Tyrosine Bifunctional 030304 developmental biology Pharmacology Tyrosine binding 0303 health sciences Binding Sites Molecular Structure Myoglobin Organic Chemistry Combinatorial chemistry 0104 chemical sciences 3. Good health chemistry Click Chemistry DNA Biotechnology Conjugate |
| Zdroj: | Bioconjugate chemistry. 24(6) |
| ISSN: | 1520-4812 |
| Popis: | We have prepared bifunctional linkers containing clickable functional groups that enable preparation of protein–DNA conjugates through binding onto tyrosine residues. Mild conjugation strategy was demonstrated using two proteins, streptavidin(STV) and myoglobin (Mb) and it resulted in conjugates with preserved functionality of both the proteins and DNA strands. Furthermore, we show that protein–DNA conjugates can be successfully immobilized onto solid surface containing complementary DNA strands and the enzymatic activity of Mb–DNA conjugates is even higher than that of corresponding conjugates prepared through Lys binding. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|