Study on Active Components of Cuscuta chinensis Promoting Neural Stem Cell Proliferation: Bioassay-Guided Fractionation

Autor:	Qiang Li, Hanze Wang, Xiao-Meng Hou, Yang Yang, Yin-Chu Si, Bing-Qi Li
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Flavonoid Drug Evaluation Preclinical Pharmaceutical Science Hyperoside Fractionation Chemical Fractionation Mass Spectrometry Article Analytical Chemistry chemistry.chemical_compound QD241-441 Chlorogenic acid Drug Discovery Animals network pharmacology Physical and Theoretical Chemistry Chromatography High Pressure Liquid Cell Proliferation neural stem cells chemistry.chemical_classification Dose-Response Relationship Drug biology Organic Chemistry Cuscuta biology.organism_classification Neural stem cell Rats Cuscuta chinensis Biochemistry chemistry Chemistry (miscellaneous) Molecular Medicine active components Female Astragalin Kaempferol Drugs Chinese Herbal
Zdroj:	Molecules Volume 26 Issue 21 Molecules, Vol 26, Iss 6634, p 6634 (2021)
ISSN:	1420-3049
DOI:	10.3390/molecules26216634
Popis:	Neural stem cells (NSCs) exist in the central nervous system of adult animals and capable of self-replication. NSCs have two basic functions, namely the proliferation ability and the potential for multi-directional differentiation. In this study, based on the bioassay-guided fractionation, we aim to screen active components in Cuscuta chinensis to promote the proliferation of NSCs. CCK-8 assays were used as an active detection method to track the active components. On the basis of isolating active fraction and monomer compounds, the structures of these were identified by LC-MS and (1H, 13C) NMR. Moreover, active components were verified by pharmacodynamics and network pharmacology. The system solvent extraction method combined with the traditional isolation method were used to ensure that the fraction TSZE-EA-G6 of Cuscuta chinensis exhibited the highest activity. Seven chemical components were identified from the TSZE-EA-G6 fraction by UPLC-QE-Orbitrap-MS technology, which were 4-O-p-coumarinic acid, chlorogenic acid, 5-O-p-coumarinic acid, hyperoside, astragalin, isochlorogenic acid C, and quercetin-3-O-galactose-7-O-glucoside. Using different chromatographic techniques, five compounds were isolated in TSZE-EA-G6 and identified as kaempferol, kaempferol-3-O-glucoside (astragalin), quercetin-3-O-galactoside (hyperoside), chlorogenic acid, and sucrose. The activity study of these five compounds showed that the proliferation rate of kaempferol had the highest effects at a certain concentration (25 μg/mL, 3.12 μg/mL), the proliferation rate could reach 87.44% and 59.59%, respectively. Furthermore, research results using network pharmacology techniques verified that kaempferol had an activity of promoting NSCs proliferation and the activity of flavonoid aglycones might be greater than that of flavonoid glycosides. In conclusion, this research shows that kaempferol is the active component in Cuscuta chinensis to promote the proliferation of NSCs.
Databáze:	OpenAIRE
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