Altered intracellular region of MUC1 and disrupted correlation of polarity-related molecules in breast cancer subtypes
| Autor: | Fumi Fuchinoue, Shinobu Masuda, Tetsuyo Maeda, Yukari Obana, Misato Iizuka, Mayumi Tani, Katsuhisa Enomoto, Sadao Amano, Eriko Murakami, Yoko Nakanishi, Kenichi Sakurai |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Cancer Research Pathology medicine.medical_specialty Receptor ErbB-2 Estrogen receptor MUC1 Breast Neoplasms Biology medicine.disease_cause Breast cancer Growth factor receptor medicine Biomarkers Tumor Humans RNA Messenger skin and connective tissue diseases Microdissection Aged intracellular localization Paraffin Embedding Mucin-1 Cell Polarity General Medicine Original Articles Middle Aged medicine.disease digestive system diseases Oncology Receptors Estrogen Cytoplasm breast cancer subtype Cancer research Female Carcinogenesis Breast carcinoma |
| Zdroj: | Cancer Science |
| ISSN: | 1349-7006 1347-9032 |
| Popis: | MUC1 glycoprotein is overexpressed and its intracellular localization altered during breast carcinoma tumorigenesis. The present study aimed to clarify the relationship of cytoplasmic localization of MUC1 with the breast cancer subtype and the correlation of 10 molecules associated with cell polarity in breast cancer subtypes. We immunostained 131 formalin-fixed and paraffin-embedded breast cancer specimens with an anti-MUC1 antibody (MUC1/CORE). For 48 of the 131 tumor specimens, laser-assisted microdissection and real-time quantitative RT-PCR were performed to analyze mRNA levels of MUC1 and 10 molecules, β-catenin, E-cadherin, claudin 3, claudin 4, claudin 7, RhoA, cdc42, Rac1, Par3 and Par6. Localization of MUC1 protein varied among breast cancer subtypes, that is, both the apical domain and cytoplasm in luminal A-like tumors (P |
| Databáze: | OpenAIRE |
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