Construction of an infectious cDNA clone of radish mosaic virus, a crucifer-infecting comovirus
| Autor: | Takuya Keima, Masayoshi Hashimoto, Takamichi Nijo, Yukari Okano, Yugo Kitazawa, Shuichiro Takahashi, Kensaku Maejima, Shigetou Namba, Ken Komatsu, Yasuyuki Yamaji |
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| Rok vydání: | 2013 |
| Předmět: |
DNA
Complementary pBluescript biology viruses Radish mosaic virus Blotting Western Comovirus food and beverages Nicotiana benthamiana Raphanus General Medicine Virus Replication biology.organism_classification Virology Virus Microscopy Electron Complementary DNA Tobacco RNA Viral Cauliflower mosaic virus Movement protein Plant Diseases |
| Zdroj: | Archives of Virology. 158:1579-1582 |
| ISSN: | 1432-8798 0304-8608 |
| Popis: | Radish mosaic virus (RaMV) is a crucifer-infecting comovirus that has been detected worldwide. Here, we report the successful construction of a full-length infectious cDNA clone of RaMV. The full-length cDNA clones corresponding to RNA1 and RNA2 of a Japanese isolate of RaMV were cloned into the pBlueScript plasmid or the binary vector pCAMBIA1301 downstream of the cauliflower mosaic virus 35S promoter. Mechanical inoculation or agroinoculation of Nicotiana benthamiana with these vectors resulted in systemic RaMV infections causing symptoms similar to those caused by the wild-type parental virus. The presence of progeny virus was verified by western blot analysis and electron microscopy. |
| Databáze: | OpenAIRE |
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