Synthesis, DNA binding and cleavage studies of Ni(II) complexes with fused aromatic N-containing ligands
| Autor: | H. S. Bhojya Naik, C.N. Sudhamani, T. R. Ravikumar Naik, M. C. Prabhakara |
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| Rok vydání: | 2009 |
| Předmět: |
Hot Temperature
Spectrophotometry Infrared Stereochemistry Intercalation (chemistry) Ligands Nucleic Acid Denaturation Cleavage (embryo) Absorption Analytical Chemistry Metal Magnetics chemistry.chemical_compound 2 2'-Dipyridyl Nickel Spectroscopy Fourier Transform Infrared Octahedral molecular geometry DNA Cleavage Instrumentation Spectroscopy Gel electrophoresis Nuclease Molecular Structure biology DNA Superhelical Viscosity Chemistry DNA Atomic and Molecular Physics and Optics Spectrophotometry visual_art biology.protein visual_art.visual_art_medium Spectrophotometry Ultraviolet pUC19 Phenanthrolines |
| Zdroj: | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 72:643-647 |
| ISSN: | 1386-1425 |
| Popis: | The three Ni(II) complexes of fused aromatic N-containing ligands such as [Ni(bnp)(3)](PF(6))(2) (1), [Ni(phen)(2)(bnp)](PF(6))(2) (2) and [Ni(bpy)(2)(bnp)](PF(6))(2) (3) (where bnp=dibenzo(b)1,8-naphthpyridine, phen=1,10-phenanthroline and bpy=bipyridine) were synthesized and structurally characterized. Elemental analysis, magnetic and spectroscopic data suggested octahedral geometry for all the complexes. Binding of these complexes with (ds)DNA were analyzed by absorption spectra, viscosity and thermal denaturation studies. Detailed analysis revealed that the metal complexes intercalates into the DNA base stack as intercalator. The oxidative cleavage activities of the complexes were studied with supercoiled (SC)pUC19 DNA by using gel electrophoresis, and the results show that complexes have potent nuclease activity. |
| Databáze: | OpenAIRE |
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