Rapid Purification of Fumonisins and Their Hydrolysis Products with Solid-Phase Extraction Columns
| Autor: | Ronald D. Plattner, Stephen M. Poling |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chromatography
Hydrolysis Extraction (chemistry) Carboxylic Acids food and beverages Fraction (chemistry) General Chemistry Mycotoxins Chromatography Ion Exchange Fumonisins Zea mays Carcinogens Environmental Solvent Acetic acid chemistry.chemical_compound Cartridge Fusarium chemistry Solid phase extraction General Agricultural and Biological Sciences Acetonitrile |
| Zdroj: | Journal of Agricultural and Food Chemistry. 47:2344-2349 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf981109h |
| Popis: | Fumonisins B(3) and B(4) (FB(3) and FB(4)) were recovered from the 50:50 acetonitrile/water extract of corn cultures of a strain of Fusarium moniliforme that does not make FB(1) or FB(2) by stirring the extract with IRA-68, a weak anion-exchange resin. The fumonisins were desorbed with 5% acetic acid in the same solvent. After dilution with water, the desorbed fumonisins were separated into FB(3) (FB(3) and FA(3)) and FB(4) (FB(4), FC(4), and FA(4)) fractions with a tC(18) solid-phase extraction (SPE) cartridge. The FB(3) fraction was then separated into FB(3) and FA(3) by using an NH(2) SPE cartridge and eluting with 5% acetic acid and increasing amounts of acetonitrile in water. Finally, FB(1) and FA(3) were hydrolyzed with calcium hydroxide. After recovery from the reaction mixture using a tC(18) cartridge, the hydrolyzed and partially hydrolyzed analogues were separated and the unreacted fumonisins recovered by using an NH(2) cartridge, initially in the normal-phase mode with increasing amounts of water in acetonitrile and then in the reversed-phase mode after the addition of 5% acetic acid to the solvent and eluting in the reverse order. |
| Databáze: | OpenAIRE |
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