Hepatocyte-derived canine familiaris-microRNAs as serum biomarkers of hepatic steatosis or fibrosis as implicated in the pathogenesis of canine cholecystolithiasis
| Autor: | Ahmed M El-Sebaey, Pavel N Abramov |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Bilirubin Gastroenterology Pathogenesis chemistry.chemical_compound Dogs Fibrosis Internal medicine Hyperlipidemia medicine Animals Dog Diseases General Veterinary business.industry Gallbladder Cholecystolithiasis Alanine Transaminase medicine.disease Fatty Liver MicroRNAs medicine.anatomical_structure chemistry Liver Pancreatitis Acute Disease Hepatocytes Alkaline phosphatase Steatosis business TBIL Biomarkers |
| Zdroj: | Veterinary clinical pathologyREFERENCES. 50 |
| ISSN: | 1939-165X |
| Popis: | BACKGROUND Hepatic cholesterol accumulation in small breed dogs is a leading risk factor for hepatic fatty changes, gallbladder hypomotility, and cholelith development, which, if not discovered early, could lead to life-threatening choledocholithiasis and acute pancreatitis. OBJECTIVE This study proposed to assess the use of hepatocyte-derived canine familiaris (cfa)-microRNAs (miRNA-122, -34a, and -21) as new diagnostic serum biomarkers of liver steatosis or fibrosis, for which both processes have been implicated in canine cholecystolithiasis. METHODS Forty client-owned dogs diagnosed with cholecystolithiasis and hepatic steatosis (C+HS) or fibrosis (C+HF) based on ultrasonographic, biochemical, and histopathologic findings, and 20 healthy dogs used as controls were included in the study. Serum cfa-miRNA expression was determined using a real-time polymerase chain reaction assay. RESULTS Serum cfa-miRNA-122 and -34a expression was significantly upregulated in the C+HS (P |
| Databáze: | OpenAIRE |
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