Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic β-Cells and Human Islets
| Autor: | Giatgen A. Spinas, Niklaus Fankhauser, Lukas Müller, Richard Züllig, Ralph Schiess, Rudolf Aebersold, Carsten Friedrich Danzer, Roger Lehmann, Sebastien Ribrioux, Bernd Wollscheid, Katrin Eckhardt, Wilhelm Krek, Alexander Schmidt |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Proteases Proteome Cell Gene Expression Biology Proteomics Biochemistry Islets of Langerhans Mice Glucagon-Like Peptide 1 Tandem Mass Spectrometry Insulin-Secreting Cells medicine Animals Humans Glucose homeostasis Receptor Cells Cultured Aged Regulation of gene expression Membrane Glycoproteins Kinase Receptor Protein-Tyrosine Kinases General Chemistry Cell biology Glucose medicine.anatomical_structure Gene Expression Regulation Immunology Protein Tyrosine Phosphatases Pancreas |
| Zdroj: | Journal of Proteome Research. 11:1598-1608 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr2007895 |
| Popis: | Cell surface N-glycoproteins provide a key interface of cells to their environment and therapeutic entry points for drug and biomarker discovery. Their comprehensive description denotes therefore a formidable challenge. The β-cells of the pancreas play a crucial role in blood glucose homeostasis, and disruption of their function contributes to diabetes. By combining cell surface and whole cell capturing technologies with high-throughput quantitative proteomic analysis, we report on the identification of a total of 956 unique N-glycoproteins from mouse MIN6 β-cells and human islets. Three-hundred-forty-nine of these proteins encompass potential surface N-glycoproteins and include orphan G-protein-coupled receptors, novel proteases, receptor protein kinases, and phosphatases. Interestingly, stimulation of MIN6 β-cells with glucose and the hormone GLP1, known stimulators of insulin secretion, causes significant changes in surface N-glycoproteome expression. Taken together, this β-cell N-glycoproteome resource provides a comprehensive view on the composition of β-cell surface proteins and expands the scope of signaling systems potentially involved in mediating responses of β-cells to various forms of (patho)physiologic stress and the extent of dynamic remodeling of surface N-glycoprotein expression associated with metabolic and hormonal stimulation. Moreover, it provides a foundation for the development of diabetes medicines that target or are derived from the β-cell surface N-glycoproteome. |
| Databáze: | OpenAIRE |
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