First genotyping of Giardia lamblia from human and animal feces in Argentina, South America
| Autor: | Marta Cecilia Minvielle, Juan Ángel Basualdo, Daniela Polverino, Nora Beatriz Molina |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Giardiasis
Male Veterinary medicine protozoal DNA parasitology genotype lcsh:QR1-502 medicine.disease_cause Polymerase Chain Reaction lcsh:Microbiology Feces Polymorphism (computer science) Risk Factors Genotype genetics Child Genetics Giardia Diarrhea Child Preschool Female medicine.symptom Polymorphism Restriction Fragment Length Triose-Phosphate Isomerase Microbiology (medical) Adult lcsh:Arctic medicine. Tropical medicine Adolescent lcsh:RC955-962 enzymology Argentina Biology Dogs genotypes medicine Giardia lamblia Animals Humans Genotyping Infant South America DNA Protozoan biology.organism_classification triosephosphate isomerase Parasitology Ciencias Médicas Cattle |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 103, Iss 1, Pp 98-103 (2008) Memórias do Instituto Oswaldo Cruz, Volume: 103, Issue: 1, Pages: 103-98, Published: FEB 2008 SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| ISSN: | 0074-0276 |
| Popis: | The purpose of this study was to investigate the genotypes of Giardia lamblia from human and animal feces and their epidemiological and clinical characteristics in Argentina, South America. Seventy isolates, 60 from humans (adults and children), eight from dogs and two from cows were processed by polymerase chain reaction-restriction fragment length polymorphism. Data corresponding to demographic, socio-cultural and environmental variables and presence/absence of signs/symptoms were collected. The triosephosphate isomerase gene was amplified from 43 (71.66%) of the 60 human fecal samples. Among these, 3/43 (6.98%) were genotype AII and 40/43 (93.02%) were genotype B. Assemblage AII was detected in three children who lived together in a shantytown and they were oligosymptomatic and none had diarrhea. This genotype was not found in animals. Genotype B showed a high prevalence in both adults and children. It was also found in polysymptomatic people, many of whom presented diarrhea. It was also found only in one dog. The present study represents the first contribution to the knowledge of G. lamblia genotypes in Argentina. Facultad de Ciencias Médicas |
| Databáze: | OpenAIRE |
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