Interferon-γ Inhibits in vitro Mobilization of Eosinophils by Interleukin-5
| Autor: | Choon-Sik Park, Yun Sung Choi, Tai Youn Rhim, Eun Nam Choi, Hun Soo Chang, Il Yup Chung, Jungsun Kim |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_treatment
Immunology Inflammation In Vitro Techniques Allergic inflammation Th2 Cells Humans Immunology and Allergy Medicine Eosinophilia Interferon gamma Phosphorylation Extracellular Signal-Regulated MAP Kinases Cell Shape Interleukin 5 business.industry General Medicine Th1 Cells Eosinophil Eosinophils Chemotaxis Leukocyte Cytokine medicine.anatomical_structure Cytokines Tumor necrosis factor alpha medicine.symptom business medicine.drug |
| Zdroj: | International Archives of Allergy and Immunology. 136:295-302 |
| ISSN: | 1423-0097 1018-2438 |
| Popis: | Background: Th2 cytokines play pivotal roles in allergic inflammation, including eosinophilia, and their actions are antagonized by Th1 cytokines, conferring them therapeutic potential. Methods: In this study, we examined the ability of a number of cytokines to suppress the activation of eosinophils that function as effector cells for allergic airway diseases. Results: Interleukin (IL)-5, IL-6, and tumor necrosis factor (TNF) induced an eosinophil shape change, whereas interferon (IFN)-γ significantly inhibited the shape change. Other cytokines, including IL-1β, IL-4, IL-10 and IL-13, had little or only slightly enhancing or reducing effects on the shape change. We further analyzed the IFN-γ effect, showing that pretreatment with IFN-γ strongly suppressed IL-5-induced eosinophil shape change, and cycloheximide (CHX) abrogated the suppression by IFN-γ, suggesting that new protein synthesis is required for the inhibitory effect by this cytokine. In agreement with these results, IFN-γ blocked the eosinophil migration and ERK phophorylation induced by IL-5, and the addition of CHX restored eosinophil chemotaxis. Conclusions: Collectively, IFN-γ may attenuate eosinophilic inflammation by directly negating eosinophil mobilization. |
| Databáze: | OpenAIRE |
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