The Use of Analgesics during Vaccination with a Live Attenuated Yersinia pestis Vaccine Alters the Resulting Immune Response in Mice
Autor: | Marilynn J. Culbreth, Melissa G. Hunter, Susan L. Welkos, David P Fetterer, Jennifer L. Shoe, Sergei S. Biryukov, Christopher P. Klimko, Alicia M. Moreau, Christopher K. Cote, Jennifer L. Dankmeyer, Raysa Rosario-Acevedo |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
mice Yersinia pestis Immunology Article immune response 03 medical and health sciences 0302 clinical medicine Immune system live vaccine Antigen Drug Discovery Medicine Pharmacology (medical) Antipyretic meloxicam acetaminophen Pharmacology Attenuated vaccine biology business.industry Antibody titer analgesia plague Vaccination Bacterial vaccine 030104 developmental biology Infectious Diseases biology.protein Antibody business 030215 immunology medicine.drug |
Zdroj: | Vaccines Volume 7 Issue 4 |
ISSN: | 2076-393X |
Popis: | The administration of antipyretic analgesics prior to, in conjunction with, or due to sequelae associated with vaccination is a common yet somewhat controversial practice. In the context of human vaccination, it is unclear if even short-term analgesic regimens can significantly alter the resulting immune response, as literature exists to support several scenarios including substantial immune interference. In this report, we used a live attenuated Yersinia pestis vaccine to examine the impact of analgesic administration on the immune response elicited by a single dose of a live bacterial vaccine in mice. Mice were assessed by evaluating natural and provoked behavior, as well as food and water consumption. The resulting immune responses were assessed by determining antibody titers against multiple antigens and assaying cellular responses in stimulated splenocytes collected from vaccinated animals. We observed no substantial benefit to the mice associated with the analgesic administration. Splenocytes from both C57BL/6 and BALB/c vaccinated mice receiving acetaminophen have a significantly reduced interferon-gamma (IFN-&gamma ) recall response. Additionally, there is a significantly lower immunoglobulin (Ig)G2a/IgG1 ratio in vaccinated BALB/c mice treated with either acetaminophen or meloxicam and a significantly lower IgG2c/IgG1 ratio in vaccinated C57BL/6 mice treated with acetaminophen. Taken together, our data indicate that the use of analgesics, while possibly ethically warranted, may hinder the accurate characterization and evaluation of novel vaccine strategies with little to no appreciable benefits to the vaccinated mice. |
Databáze: | OpenAIRE |
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