Diversity of pathogenic Pseudomonas isolated from citrus in Tunisia

Autor: Maroua Oueslati, Mohamed Rabeh Hajlaoui, Odile Berge, Magdalena Mulet, Najla Sadfi-Zouaoui, Elena García-Valdés, Jorge Lalucat, Charlotte Chandeysson, Mohamed Zouaoui
Přispěvatelé: Université de Tunis El Manar (UTM), Universitat de les Illes Balears (UIB), Unité de Pathologie Végétale (PV), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Mediterrani d'Estudis Avancats (IMEDEA), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)-Universidad de las Islas Baleares (UIB), Université de Carthage - University of Carthage, Institut National de la Recherche Agronomique de Tunisie (INRAT), Spanish MINECO (CGL2015-70925), co-founded by the European Regional Development Fund/European Social Fund 'Investing in your future' (FEDER), PRF Project (PRF14CTABIOTEC02), Ministry of Higher Education and Scientific Research of Tunisia (LR16ES05)., Ministerio de Economía y Competitividad (España), European Commission, Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie)
Rok vydání: 2020
Předmět:
0106 biological sciences
Citrus
lcsh:Biotechnology
Cultivars
lcsh:QR1-502
[SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy
Biophysics
Pseudomonas syringae
Pseudomonas congelans
culture fruitière
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics
Phylogenetics and taxonomy
01 natural sciences
Applied Microbiology and Biotechnology
lcsh:Microbiology
Microbiology
03 medical and health sciences
lcsh:TP248.13-248.65
Pathogenicity
Cultivar
Gene
030304 developmental biology
0303 health sciences
biology
Phylogenetic tree
Pseudomonas
Pseudomonas cerasi
biology.organism_classification
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
3. Good health
agrume
Original Article
rpoD
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology
environment/Symbiosis
010606 plant biology & botany
Zdroj: AMB Express
AMB Express, Springer Nature, 2020, 10, ⟨10.1186/s13568-020-01134-z⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
AMB Express, Vol 10, Iss 1, Pp 1-20 (2020)
ISSN: 2191-0855
Popis: The damages observed in Tunisian citrus orchards have prompted studies on the Pseudomonas spp. responsible for blast and black pit. Prospective orchards between 2015 and 2017 showed that the diseases rapidly spread geographically and to new cultivars. A screening of Pseudomonas spp. isolated from symptomatic trees revealed their wide diversity according to phylogenetic analysis of their housekeeping rpoD and cts genes. The majority of strains were affiliated to Pseudomonas syringae pv. syringae (Phylogroup PG02b), previously described in Tunisia. However, they exhibited various BOX-PCR fingerprints and were not clonal. This work demonstrated, for the first time in Tunisia, the involvement of Pseudomonas cerasi (PG02a) and Pseudomonas congelans (PG02c). The latter did not show significant pathogenicity on citrus, but was pathogenic on cantaloupe and active for ice nucleation that could play a role in the disease. A comparative phylogenetic study of citrus pathogens from Iran, Montenegro and Tunisia revealed that P. syringae (PG02b) strains are closely related but again not clonal. Interestingly P. cerasi (PG02a) was isolated in two countries and seems to outspread. However, its role in the diseases is not fully understood and it should be monitored in future studies. The diversity of pathogenic Pseudomonas spp. and the extension of the diseases highlight that they have become complex and synergistic. It opens questions about which factors favor diseases and how to fight against them efficiently and with sustainable means.
This study was funded by the Spanish MINECO (CGL2015-70925), co-founded by the European Regional Development Fund/European Social Fund “Investing in your future” (FEDER). This work was also supported by funds from the PRF Project (PRF14CTABIOTEC02) and by the Ministry of Higher Education and Scientific Research of Tunisia (LR16ES05).
Databáze: OpenAIRE
Externí odkaz: