Unveiling conformational dynamics changes of H-Ras induced by mutations based on accelerated molecular dynamics
| Autor: | Laixue Pang, Weiliang Zhu, Wei Wang, Jianzhong Chen |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Conformational change
Protein Conformation General Physics and Astronomy Plasma protein binding Molecular Dynamics Simulation medicine.disease_cause 01 natural sciences Proto-Oncogene Proteins p21(ras) 03 medical and health sciences Closed state Molecular dynamics Protein structure 0103 physical sciences medicine Humans Physical and Theoretical Chemistry Pliability 030304 developmental biology Guanylyl Imidodiphosphate Principal Component Analysis 0303 health sciences Mutation 010304 chemical physics Chemistry Dynamics (mechanics) Biophysics Thermodynamics Protein Binding |
| Zdroj: | Physical Chemistry Chemical Physics. 22:21238-21250 |
| ISSN: | 1463-9084 1463-9076 |
| Popis: | Uncovering molecular basis with regard to the conformational change of two switches I and II in the GppNHp (GNP)-bound H-Ras is highly significant for the understanding of Ras signaling. For this purpose, accelerated molecular dynamics (aMD) simulations and principal component (PC) analysis are integrated to probe the effect of mutations G12V, T35S and Q61K on conformational transformation between two switches of the GNP-bound H-Ras. The RMSF and cross-correlation analyses suggest that three mutations exert a vital effect on the flexibility and internal dynamics of two switches in the GNP-bound H-Ras. The results stemming from PC analysis indicate that two switches in the GNP-bound WT H-Ras tend to form a closed state in most conformations, while those in the GNP-bound mutated H-Ras display transformation between different states. This conclusion is further supported by free energy landscapes constructed by using the distances of residues 12 away from 35 and 35 away from 61 as reaction coordinates and different experimental studies. Interaction scanning is performed on aMD trajectories and the information shows that conformational transformations of two switches I and II induced by mutations extremely affect the GNP-residue interactions. Meanwhile, the scanning results also signify that residues G15, A18, F28, K117, A146 and K147 form stable contacts with GNP, while residues D30, E31, Y32, D33, P34 and E62 in two switches I and II produce unstable contacts with GNP. This study not only reveals dynamic behavior changes of two switches in H-Ras induced by mutations, but also unveils general principles and mechanisms with regard to functional conformational changes of H-Ras. |
| Databáze: | OpenAIRE |
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