Production and characterization of monoclonal antibodies against α-globin chain-containing human hemoglobins for detecting α-thalassemia disease

Autor:	Sawitree Chiampanichayakul, Thanusak Tatu, Kanet Pakdeepak, Watchara Kasinrerk, Supansa Pata
Rok vydání:	2016
Předmět:	Indirect elisa medicine.drug_class Lymphocyte Thalassemia Hydrops Fetalis Clinical Biochemistry Immunology Spleen Enzyme-Linked Immunosorbent Assay Biology Monoclonal antibody α globin 03 medical and health sciences 0302 clinical medicine alpha-Globins alpha-Thalassemia Antibody Specificity hemic and lymphatic diseases Hydrops fetalis medicine Immunology and Allergy Humans Antibodies Monoclonal medicine.disease Molecular biology Medical Laboratory Technology medicine.anatomical_structure 030220 oncology & carcinogenesis Hemoglobin 030215 immunology
Zdroj:	Journal of immunoassayimmunochemistry. 37(6)
ISSN:	1532-4230
Popis:	Monoclonal antibodies against α-globin containing human Hbs, named AMS-Alpha1 and AMS-Alpha 2, were produced by the hybridoma technique using spleen cells enriched by the newly developed B lymphocyte enrichment protocol. These two monoclonal antibodies were of IgM class, reacting to only intact form of human Hbs A, A2, E, and F, which contain α-globin chain. By the indirect ELISA, the AMS-Alpha1 and AMS-Alpha 2 quantified less amount of α-globin chain containing hemoglobins in HbH disease than the SEA-α thalassemia 1 carriers and normal individuals. It was thus anticipated that these monoclonal antibodies can be used for detecting Hb Bart's hydrops fetalis in which no α-globin chain is produced.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10cfa8bdfbc7f973c3dbf5ee1b011ad4 https://pubmed.ncbi.nlm.nih.gov/27050832 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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