Biochemical and histochemical properties of hepatic tumors of rainbow trout, Oncorhynchus mykiss
| Autor: | Lynn M. Parker, Bruce S. Winder, David E. Hinton, Darrel J. Laurén, Bruce D. Hammock |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cancer Research
Aflatoxin B1 Trout Liver cytology Aldehyde dehydrogenase Dehydrogenase Uridine Diphosphate Glucose Dehydrogenase chemistry.chemical_compound Cytochrome P-450 Enzyme System Cytochrome P-450 CYP1A1 NAD(P)H Dehydrogenase (Quinone) medicine Animals Glucuronosyltransferase Epoxide hydrolase Glutathione Transferase biology Liver Neoplasms gamma-Glutamyltransferase General Medicine Aldehyde Dehydrogenase Immunohistochemistry Uridine Disease Models Animal medicine.anatomical_structure Glutathione S-transferase Liver Biochemistry chemistry Hepatocyte biology.protein Oxidoreductases |
| Zdroj: | Carcinogenesis. 14:211-217 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/14.2.211 |
| Popis: | Biochemical and histochemical studies were conducted in aflatoxin B1-induced liver tumors in adult rainbow trout. Specific activities of the phase I enzymes, ethoxyresorufin-O-deethylase (EROD), microsomal and cytosolic epoxide hydrolase (mEH and cEH), aldehyde dehydrogenase (ALDH) and DT-diaphorase, and the phase II enzymes, gamma-glutamyltransferase (gamma-GT), glutathione transferase (GST) and uridine diphosphoglucuronyl transferase (UDPGT) were measured. Cryostat sections of tumor and surrounding liver from the same cohorts were analyzed immunohistochemically for cytochrome P450IA1 and histochemically for ALDH (benzaldehyde and hexanal), DT-diaphorase, gamma-GT and uridine diphosphoglucuronyl dehydrogenase (UDPGdH). In tumor tissues, the largest biochemical changes were found with benzaldehyde dehydrogenase, where activity increased from undetectable levels to 7.4 nmol/min/mg protein, and gamma-GT, where activity increased 12-fold over controls. Increases in other enzymes ranged from 1.26 to 2.84 times that of control liver, except EROD, which decreased, and cEH and mEH, which were unchanged. Histochemical analyses showed the induction of ALDH, gamma-GT, DT-diaphorase and UDPGdH, and the depression of cytochrome P450IA1 in hepatic neoplasms. In addition, marker enzyme histochemistry of neoplasms revealed heterogeneous populations of hepatocytes and absence of necrotic areas. |
| Databáze: | OpenAIRE |
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