miR-143 suppresses the osteogenic differentiation of dental pulp stem cells by inactivation of NF-κB signaling pathway via targeting TNF-α
| Autor: | Peng Zhang, Wenli Yang, Guo-fang Wang, Ya-jing Li |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adolescent Blotting Western Cell Bone Morphogenetic Protein 2 Core Binding Factor Alpha 1 Subunit Enzyme-Linked Immunosorbent Assay Real-Time Polymerase Chain Reaction Bone morphogenetic protein 2 Young Adult 03 medical and health sciences 0302 clinical medicine stomatognathic system Western blot Osteogenesis Dental pulp stem cells medicine Humans General Dentistry Transcription factor Dental Pulp medicine.diagnostic_test Tumor Necrosis Factor-alpha Chemistry Stem Cells NF-kappa B Cell Differentiation Cell Biology General Medicine Alkaline Phosphatase Cell biology RUNX2 MicroRNAs 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology 030220 oncology & carcinogenesis Dentinogenesis Signal transduction Biomarkers Signal Transduction |
| Zdroj: | Archives of Oral Biology. 87:172-179 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2017.12.031 |
| Popis: | Background Dental pulp stem cells (DPSCs) are multipotent and play an important role in repairing damaged and/or defective dentinogenesis/osteogenesis. Recent studies have documented the implication of miR-143 in osteogenic differentiation of DPSCs. Nevertheless, the detailed mechanisms of miR-143 involved in the osteogenic differentiation of DPSCs remain to be further elaborated. Methods Isolated DPSCs were incubated with osteogenic differentiation medium to induce osteogenic differentiation. qRT-PCR and western blot were performed to determine the expressions of miR-143 and tumor necrosis factor α (TNF-α). Luciferase reporter assay was used to confirm whether TNF-α was a target of miR-143. Osteogenic differentiation of DPSCs was evaluated by alkaline phosphatase (ALP) activity assay, ALP staining, and western blot analyses of osteogenic-markers including bone morphogenetic protein 2 (BMP2), ALP, runt-related transcription factor 2 (RUNX2) and collagen type I (COLI). Results miR-143 was downregulated and TNF-α was upregulated during osteogenic differentiation of DPSCs. miR-143 posttranscriptionally regulated TNF-α expression in DPSCs by binding to its 3′UTR. miR-143 overexpression suppressed osteogenic differentiation of DPSCs, as demonstrated by the decrease of ALP activity, ALP positive cell ratio, as well as BMP2, ALP, RUNX2, and COLI expressions. Moreover, miR-143 reversed TNF-α-induced osteogenic differentiation of DPSCs. Finally, the osteogenic differentiation of DPSCs induced by miR-143 inhibitor was attenuated following inactivation of nuclear factor kappa B (NF-κB) signaling pathway. Conclusion miR-143 suppressed the osteogenic differentiation of DPSCs by blockade of NF-κB signaling pathway via targeting TNF-α. |
| Databáze: | OpenAIRE |
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