LHX6, An Independent Prognostic Factor, Inhibits Lung Adenocarcinoma Progression through Transcriptional Silencing of β-catenin
| Autor: | Huidong Zhang, Xiao Jiang, Yongsheng Huang, Xiang-Lin Hao, Fei Han, Jia Cao, Jinyi Liu, Li Yin, Jun-Tang Yang, Ming-qian Zhang, Hongqiang Chen, Wen-bin Liu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.disease_cause Metastasis 03 medical and health sciences 0302 clinical medicine LHX6 medicine Gene silencing prognostic factor Lung cancer Lymph node β-catenin Tissue microarray business.industry lung adenocarcinoma medicine.disease Squamous carcinoma 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Adenocarcinoma business Carcinogenesis Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| DOI: | 10.7150/jca.19972 |
| Popis: | Introduction: Our previous study identified LIM homeobox domain 6 (LHX6) as a frequently epigenetically silenced tumor-suppressor gene in lung cancer. However, its clinical value has never been evaluated, and the in-depth anti-tumor mechanism remains unclear. Methods: Public database was used for lung cancer, lung adenocarcinoma and lung squamous carcinoma patients and tissue microarray data was used for lung adenocarcinoma patients to study prognostic outcome of LHX6 expression by Kaplan-Meier and Cox-regression analysis. In vitro proliferation, metastasis and in vivo nude mice model were used to evaluate the anti-tumor effect of LHX6 on lung adenocarcinoma cell lines. The mechanisms were explored using western blot, TOP/FOP flash assays and luciferase reporter assays. LHX6 expression and clinical stages data were collected from The Cancer Genome Atlas database (TCGA). Results: Expression of LHX6 was found to be a favorable independent prognostic factor for overall survival (OS) of total lung adenocarcinoma patients (P=0.014) and patients with negative lymph nodes status (P=0.014) but not related the prognostic outcome of lung squamous cell carcinoma patients. The expression status of LHX6 significantly correlated to histological grade (P |
| Databáze: | OpenAIRE |
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