P25 and P28 proteins of the malaria ookinete surface have multiple and partially redundant functions
| Autor: | Pietro Lupetti, Gabriele Margos, Chris J. Janse, Maria del Carmen Rodriguez, George Dimopoulos, Geoffrey Butcher, Marianna Karras, Tania F. de Koning-Ward, Annette L. Beetsma, Ana M. Tomás, Leo H.M. van Lin, Jacqui Mendoza, Andrew P. Waters, Ria Sinha, Robert E. Sinden, Fotis C. Kafatos, Ariadne Hager |
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| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
PARASITE PLASMODIUM-FALCIPARUM
Plasmodium berghei medicine.medical_treatment Protozoan Proteins Antigens Protozoan TARGETED DISRUPTION Plasmodium MOSQUITO ANOPHELES-GAMBIAE EPIDERMAL GROWTH-FACTOR General Biochemistry Genetics and Molecular Biology SEXUAL STAGES Article Epithelium Antigen INFECTION Anopheles parasitic diseases medicine TRANSMISSION-BLOCKING ANTIBODIES Animals AEDES-AEGYPTI MOSQUITOS BERGHEI GALLINACEUM Molecular Biology Gene Protease General Immunology and Microbiology biology General Neuroscience fungi Midgut biology.organism_classification medicine.disease Virology Cell biology Antigens Surface Digestive System Malaria |
| Zdroj: | The EMBO journal. 20(15) |
| ISSN: | 1460-2075 0261-4189 |
| Popis: | The ookinete surface proteins (P25 and P28) are proven antimalarial transmission-blocking vaccine targets, yet their biological functions are unknown. By using single (Sko) and double gene knock-out (Dko) Plasmodium berghei parasites, we show that P25 and P28 share multiple functions during ookinete/oocyst development. In the midgut of mosquitoes, the formation of ookinetes lacking both proteins (Dko parasites) is significantly inhibited due to decreased protection against lethal factors, including protease attack. In addition, Dko ookinetes have a much reduced capacity to traverse the midgut epithelium and to transform into the oocyst stage. P25 and P28 are partially redundant in these functions, since the efficiency of ookinete/oocyst development is only mildly compromised in parasites lacking either P25 or P28 (Sko parasites) compared with that of Dko parasites. The fact that Sko parasites are efficiently transmitted by the mosquito is a compelling reason for including both target antigens in transmission-blocking vaccines. |
| Databáze: | OpenAIRE |
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